Differential splicing of the lectin domain of an O-glycosyltransferase modulates both peptide and glycopeptide preferences

Mucin-type O-glycosylation is an essential post-translational modification required for protein secretion, extracellular matrix formation and organ growth. O-glycosylation is initiated by a large family of enzymes (GALNTs in mammals and PGANTs in Drosophila ) that catalyze the addition of N-acetylgalactosamine (GalNAc) onto the hydroxyl groups of serines or threonines

GalNAc, while the lectin domain recognizes more distant extant GalNAc on previously glycosylated substrates. We previously demonstrated a novel role for the a repeat of lectin domain in influencing charged peptide preferences. Here, we further interrogate how the differentially spliced a repeat of the PGANT9A and PGANT9B Oglycosyltransferases confers distinct preferences for a variety of endogenous substrates. Through biochemical analyses and in silico modeling using preferred substrates, we find that a combination of charged residues within the a repeat and charged residues in the flexible gating loop of the catalytic domain distinctively influence the peptide substrate preferences of each splice variant. Moreover, PGANT9A and PGANT9B also display unique glycopeptide preferences. These data illustrate how changes within the non-catalytic lectin domain can alter the recognition of both peptide and glycopeptide substrates. Overall, our results elucidate a novel mechanism for modulating substrate preferences of O-glycosyltransferases via alternative splicing within specific subregions of functional domains. ________________________________________ Proteins undergo post-translational modifications (PTMs) that regulate their structure, stability, function, and interaction with other molecules. Mucin-type O-glycosylation is an evolutionarily conserved PTM that is implicated in human health and disease (reviewed in (1,2)). Indeed, the disease hyperphosphatemic familial tumoral calcinosis (HFTC), which is typified by hyperphosphatemia and the development of subdermal calcified tumors, is caused by mutations in the GALNT3 gene, whose product is responsible for the initiation of O-glycosylation (3)(4)(5). Additionally, changes in O-glycosylation are also associated with other conditions, including blood lipid levels, bone density, kidney function, and the development and progression of certain types of cancer (5)(6)(7)(8)(9)(10)(11)(12)(13). More recently, congenital loss of GALNT2 function has been found to underlie a complex neurological syndrome (14). Therefore, a comprehensive understanding of the factors that regulate the synthesis and function of O-glycans is crucial to understanding how their alteration contributes to disease.
Biogenesis of mucin-type O-glycosylation is initiated in the Golgi through the transfer of a GalNAc monosaccharide to serine or threonine residues of target proteins. A large family of UDP-GalNAc:polypeptide α-Nacetylgalactosaminyltransferases (i.e.,Galnts or GALNTs) initiates this first sugar addition (~20 Galnts in mammals and 10 PGANTs in Drosophila) (1,2). Members of this family show unique spatial and temporal expression patterns during embryonic development and different expression levels in various organs and tissues, suggesting that each member has unique in vivo substrates and functional roles (1,2,15,16). Structurally, members of this family are similar, with each having a conserved catalytic domain responsible for binding donor (UDP-GalNAc) and acceptor (proteins or peptides) substrates to coordinate GalNAc transfer, as well as a separate ricin-like lectin domain that potentially recognizes an extant GalNAc on previously glycosylated substrates (17,18). The catalytic domain contains a catalytic flexible gating loop that becomes ordered and adopts a closed conformation upon acceptor and donor substrate binding (19)(20)(21)(22)(23). The lectin-domain is unique to the GALNT enzyme family and consists of 3 repeats (a, b, g) that can potentially bind to previously glycosylated substrates. Such binding positions the acceptor site of a glycopeptide substrate into the catalytic domain to facilitate the glycosylation of additional, more distant sites (17)(18)(19)(21)(22)(23)(24)(25). Crystal structures of GALNT2, GALNT3, GALNT4 and GALNT12 bound to glycopeptide substrates show the glycopeptide-Thr-GalNAc bound to the a repeat of the lectin domain in an identical manner to help position the acceptor threonine into the active site of the catalytic domain at a distance (5-17 residues) from the lectin bound glycopeptide-Thr-GalNAc (19)(20)(21)(22).
Studies employing libraries of peptides have revealed unique amino acid preferences vicinal to the site of glycosylation for the catalytic domains of several family members (26,27). Likewise, libraries of glycopeptide substrates have revealed isoformspecific preferences for the position of extant GalNAc residues (relative to sites of glycosylation) within the lectin domain (17,25). Specifically, certain family members prefer that the extant remote GalNAc is C-terminal to the putative site of GalNAc addition (GALNT1, -T2 and -T14), while  others prefer the extant remote GalNAc to be Nterminal to the site of GalNAc addition (GALNT3,  -T4, -T6 and -T12) (17,25). This selectivity is in part influenced by changes in the ~10 amino acid flexible linker between the catalytic and lectin domains (24). Taken together, the distinctive structural features of each family member are thought to confer specific peptide and glycopeptide preferences to ensure the correct modification of unique sites, resulting in the dense but deliberate glycosylation of mucins and mucin-like proteins that consist of regions rich in serine and threonine. The high density of O-glycans found in mucins is thought to confer many biological properties, such as resistance to proteolysis, rigid rod like structures, and the ability to form highly protective, hydrated membranes along many epithelial surfaces of the body (28)(29)(30).
Studies in model organisms have demonstrated that O-glycosylation is essential for viability (16,31,32), and has roles in extracellular matrix (ECM) formation and secretion, cell adhesion, cell proliferation, microbiome composition and organ growth and development (30,33-37). Previous work in the fruit fly Drosophila melanogaster, identified a member of this enzyme family (CG30463; pgant9) that influenced viability (16). Recent studies have found that pgant9 is differentially spliced within the Drosophila salivary gland to generate 2 isoforms (PGANT9A and PGANT9B) that differ only within the a repeat of the lectin region (38). Moreover, the expression of each isoform has differential effects on the morphology of secretory granules within the salivary gland, due to differential glycosylation of an in vivo mucin (38). Here, we further characterize the substrate specificities of PGANT9A and PGANT9B in vitro. Interestingly, we find that PGANT9A and PGANT9B have differential preferences for charged peptide substrates that are dictated by charged residues within the a repeat along with charged residues within the gating loop of the catalytic domain. Additionally, we find that each splice variant displays unique glycopeptide specificities, indicating that this lectin domain splicing event further influences recognition of glycopeptide substrates. Our results provide the first demonstration of a developmentally-regulated splicing event within the lectin domain of a GALNT/PGANT family member that can modulate both peptide and glycopeptide substrate preferences.
These studies provide a new regulatory mechanism by which the specificity of O-glycosylation can be modulated in vivo and coordinated with the expression of endogenous substrates.

Results
Alternative splicing of the lectin domain a repeat alters mucin peptide preferences pgant9 is differentially spliced in a tissuespecific manner that changes only the a repeat of the lectin region ( Fig. 1A) to yield 2 splice variants known as pgant9A and pgant9B (38). This splicing event results in an a repeat that is highly positively charged (PGANT9A) or highly negatively charged (PGANT9B) (Fig. 1B) (38). Previous work has shown that each splice variant exhibited a preference for an oppositely charged peptide substrate (38).
In that study, the negatively charged PGANT9B preferred to glycosylate the positively charged Sgs3 mucin, and the specific loss of PGANT9B resulted in defects in secretory granule morphology, likely due to alterations in the glycosylation of Sgs3. PGANT9A showed little activity on the positively charged region of Sgs3, but robustly glycosylated this region when positively charged amino acids were changed to negatively charged ones (38). This led to the suggestion that this splicing event may exist to by guest on November 3, 2020 http://www.jbc.org/ Downloaded from ensure proper glycosylation of endogenous substrates containing charged regions. To further explore whether PGANT9A and PGANT9B have distinct preferences for other endogenous substrates, we examined their activity toward a variety of mucins expressed in Drosophila (Fig. 1C-D and Fig.  S1). We created peptide substrates based on the repetitive regions unique to each mucin and performed enzymatic assays using purified PGANT9A and PGANT9B expressed in Pichia pastoris (38). As shown in Fig. 1C, both PGANT9A and PGANT9B glycosylated a number of mucin-based peptides, including Muc18B, Muc11A, Sgs1, and Muc55B, in addition to the previously characterized Sgs3. Based on these results, we next tested whether PGANT9A and PGANT9B preferences for substrates containing charged residues could be predictably altered by varying peptide charge. We therefore changed each charged residue to an oppositely charged residue and performed in vitro enzyme assays ( Fig As shown previously, PGANT9B robustly glycosylates the endogenous Sgs3 substrate (which is positively charged) but has reduced acivity on the negatively charged version (Sgs3-E) ( Fig. 2A and (38)).
In contrast, PGANT9A prefers the negatively charged version (Sgs3-E) and does not act substantially on the positively charged Sgs3 ( Fig. 2A and (38)). Similarly, Sgs1 (which is preferred by PGANT9B), can become a more robust substrate for PGANT9A by changing positively charge residues to negatively charged ones (Sgs1-E) ( Fig. 2B and Fig.  S2). Interestingly however, this change had little effect on the activity of PGANT9B ( Fig. 2B and Fig.  S2). Likewise, the preference of PGANT9A for Muc18B or Muc11A was dramatically reduced when negatively charged residues were changed to positively charged ones (Muc18B-K or Muc11A-K) ( Fig. 2C-D). However, the activity of PGANT9B was not as dramatically altered with these charged residue changes (Fig.2C-D and Fig. S2). Taken together, our results provide evidence that PGANT9A has a strong preference for negatively charged mucin-based substrates and that this preference can be predictably altered by altering substrate charge. In contrast, the preference of PGANT9B for positively charged substrates was not as striking.
To further understand why PGANT9A is apparently more sensitive to substrate charge than PGANT9B, we next examined which charged residues have the most influence on the activities of PGANT9A or PGANT9B. Using a peptide based on the previously characterized Sgs3 mucin sequence (which contains regularly spaced charged residues every 5 amino acids), we altered each charged residue individually and then tested PGANT9A or PGANT9B activity ( PGANT9A activity predictably increased with the addition of each negatively charged residue at each position in the peptide substrate (Fig. 3A). Unexpectedly, PGANT9B activity increased dramatically with the addition of a negative residue at amino acid position 5 in the peptide (Fig. 3B). These results suggest that a negative charge at this particular position is somehow increasing the ability of PGANT9B to glycosylate this substrate, even though the a repeat of PGANT9B is negatively charged.
To understand how this might be happening, we designed peptides based on the Sgs3 sequence with only one acceptor site to see where PGANT9A or PGANT9B prefer to glycosylate ( Fig. 4 and Fig.  S4).
Based on these single site acceptors, PGANT9A or PGANT9B will glycosylate a threonine in the center of the peptide (threonine 8) or near the N-terminus (threonine 3). Neither splice variant acted on a threonine at the C-terminus (threonine 13) ( Fig. 4A and B). To further explore substrate preference, we next examined which prolines influenced enzymatic activity on single site acceptors. Previous studies have shown that most GALNTs have a conserved proline binding pocket within the active site that interacts with prolines at the +1 and +3 position of the acceptor threonine to coordinate substrate binding (25,26). Indeed, the previously solved structures of PGANT9A and PGANT9B also predict such a binding pocket exists within their catalytic domains (38). To test this, we mutated each proline in the Sgs3-AT8 single site acceptor and performed assays with PGANT9A or PGANT9B. While PGANT9A showed no activity against any of these substrates (likely due to its very low activity against the Sgs3-AT8 single site acceptor), PGANT9B still had robust activity against substrates in which the Nterminal (proline 1) or central proline (proline 6) were changed to alanine ( However, PGANT9B activity was completely abrogated when the C-terminal proline (proline 11) was mutated ( Fig. 4C and D). These results suggest that this proline 11 likely sits in the proline pocket of PGANT9B to position threonine 8 in the catalytic center for glycosylation.

In silico modeling reveals contributions of the a repeat and gating loop in influencing charged peptide preferences
Based on this in vitro data, we modeled the Sgs3 peptide and the single charge variant (Sgs3-1E5) into the crystal structure of PGANT9B using threonine 8 as the acceptor and placing the +3 proline (proline 11) in the conserved proline binding pocket ( Fig. 5A and B). We also modeled the negatively charged variant (Sgs3-E, which PGANT9A prefers) into PGANT9A using the same parameters (Fig. 5C). Interestingly, these models predict that a positively charged region (AA 437-450) in the catalytic flexible gating loop of PGANT9A and PGANT9B ( Fig. 5D and E) will lie in close proximity to the N-terminus of the peptide substrate ( Fig. 5A-C). Within that region are 4 consecutive residues that contain a net positive charge in most human and fly isoforms, corresponding to RKRS in PGANT9A and PGANT9B (AA 440-443) ( Fig. 5D and E). However, PGANT9A and PGANT9B also contain additional non-conserved positively-charged residues (Lys446 and Arg448) within this gating loop (starred residues in Fig. 5E). The net positive charge in the gating loop may explain why a negative charge in the N-terminus of Sgs3-1E5 would enhance activity for PGANT9B (Fig. 5A vs  B), independent of the negative charges within its lectin domain a repeat, which are predicted to interact with the C-terminus of the peptide. Likewise, modeling Sgs3-E into the crystal structure of PGANT9A shows the negatively charged peptide interacting with both the positively charged gating loop and the positively charged a repeat (Fig. 5C). Taken together, these results support a more complex model where the substrate preferences of PGANT9A and PGANT9B are further influenced by a positively charged region within the catalytic gating loop. The combination of the positive charge in the gating loop along with the positive charge of the a repeat are likely responsible for the strong preference for negatively charged substrates seen for PGANT9A. Likewise, the combination of positive charge in the gating loop combined with negative charge in the a repeat of PGANT9B may explain why it is less sensitive to substrate charge.
To further test this model, we created a series of peptide substrates that varied in charge at positions N-terminal and C-terminal to the site of glycosylation (Fig. 6A) to interrogate PGANT9A and PGANT9B charge preferences.
The Nterminal and C-terminal regions contained either neutral (N), acidic (A) or basic (B) patches flanking the acceptor threonine (Fig. 6A). This array of nine peptide variants was incubated with either PGANT9A or PGANT9B and glycosylation was quantified ( Fig. 6B and C).
Interestingly, PGANT9A strongly preferred peptides that contained acidic regions N-and/or C-terminal to the site of GalNAc addition, with no basic regions present ( Fig. 6B and Fig. S5A). PGANT9B preferred peptides with a C-terminal basic charge that also had an acidic or neutral region in the Nterminus ( Fig. 6C and Fig. S5B). These results are in agreement with our in silico modeling shown in Fig. 5 and in vitro data on endogenous substrates, and provide further support for a model where charge within the gating loop of the catalytic domain combines with the charge of the a repeat of the lectin domain to drive peptide substrate preferences.

Alternative lectin domain a repeats confer unique glycopeptide substrate preferences
We next examined whether the differential splicing within the a repeat of the lectin domain of PGANT9A and PGANT9B altered their preferences for glycopeptide substrates. Previous studies have shown that the lectin domain is able to recognize extant GalNAc on glycopeptide substrates to influence further GalNAc addition by the catalytic domain (17,18,25). To test whether splicing within the a repeat of the lectin domain also influences glycopeptide preferences, we performed enzyme assays on glycopeptide substrates ( Fig. 7 and Fig. S6). We noticed consistent and reproducible differences in the activities of PGANT9A and PGANT9B on certain glycopeptide substrates relative to the unglycosylated MUC5AC peptide ( Fig. 7A and B), suggesting that this splicing event may further influence glycopeptide affinity/preferences. We therefore characterized the glycopeptide preferences of each splice variant using a series of universal randomized glycopeptide substrate libraries designed to probe the role of the lectin domain in recognizing prior remote GalNAc glycosylation (25,26). These random glycopeptide substrates contain a single GalNAc residue placed near the N-or C-terminal and a distant region of randomized residues, 5-17 residues away, which also contain Thr as an acceptor (Fig. 8A) (17). As shown in Fig. 8B and C, both PGANT9A and PGANT9B show significant enhanced activity against both the N-and Cterminal glycopeptides (GP(T*22)R or GP(T*10)L) compared to the nonglycosylated peptides (GP(A22)R or GP(A10)L). This indicates that both N-and C-terminal extant GalNAc residues are recognized by both PGANT9A and PGANT9B lectin domains to enhance the ability of the catalytic domain to transfer GalNAc to more distant sites. Interestingly, PGANT9A and PGANT9B displayed slightly different preferences for the orientation of the glycosylated residue relative to the site where the catalytic domain would transfer additional GalNAc (Fig.8B-E). PGANT9B slightly prefers glycopeptide substrates where the extant GalNAc is C-terminal to the sites of glycosylation (GP(T*22)R) while PGANT9A slightly prefers glycopeptide substrates where the extant GalNAc is N-terminal to the sites of GalNAc addition (GP(T*10)L ) (Fig. 8B-D). This difference is expressed as a ratio in Fig. 8D and as a schematic drawing in Fig. 8E. These data suggest that the splicing event within the a repeat of the lectin domain of PGANT9A and PGANT9B also generates unique glycopeptide substrate preferences. Taken together, our study provides the first evidence for developmentally-regulated alternative splicing within the lectin domain of a member of the polypeptide GalNAc transferase family that influences both peptide and glycopeptide substrate specificity.

Discussion
Here we provide the first demonstration that a developmentally regulated splicing event controls multiple aspects of substrate preferences within members of the polypeptide GalNAc transferase family. Specifically, we show that splicing within the non-catalytic portion of the enzyme influences both peptide and glycopeptide preferences. Oglycosylation is an essential protein modification that is catalyzed by a large family of enzymes that are each thought to have some overlapping as well as unique substrate specificities (1,2). The identification of splicing as another mechanism to further refine the substrate preferences of an individual isoform adds a new layer of complexity to the factors that govern this essential posttranslational modification.
In this study, we provide new mechanistic details of how splicing in the non-catalytic lectin domain can alter peptide preferences.
Our previous work suggested a model where the positively charged a repeat of PGANT9A, which lies in close proximity to the active site, allowed access of negatively charged substrates while the negatively charged a repeat of PGANT9B allowed access of positively charged substrates (38). This model was supported by the preferences of each splice variant for one mucin based peptide, Sgs3 (38). However, our studies herein interrogating the preferences for other endogenous substrates revealed a more complicated story.
While PGANT9A still displayed a striking preference for negatively charged peptide substrates, PGANT9B was less sensitive to substrate charge. In silico modeling of the preferred substrates for each splice variant highlighted a role for charged residues within the catalytic region of each enzyme in dictating substrate preference. In particular, a cluster of positively charged residues in the flexible catalytic gating loop of both enzymes are predicted to lie in close proximity to the N-terminus of substrates docked within the active site and thus influence substrate preferences. These models predicted that the positive charges of the gating loop combined with the positive charges of the a repeat of the lectin domain of PGANT9A would result in a strong preference for negatively charged substrates as well as a strong aversion to positively charged substrates.
Likewise, the positive charges of the gating loop combined with the negative charges of the a repeat of PGANT9B would result in an enzyme that could interact with substrates that contained both positively and negatively charged regions. Indeed, these models were supported by our in vitro data using peptides derived from endogenous mucin substrates. Furthermore, synthetic peptide substrates with varied charge N-and C-terminal to the site of GalNAc addition confirmed these predictions; PGANT9A strongly preferred negatively charged amino acids in both N-and C-terminal regions, while PGANT9B preferred negative charges in the N-terminus and positive charges in the C-terminus. Taken together, our biochemical and in silico modeling data supports a model where charge within the gating loop of the catalytic domain combines with charge of the a repeat of the lectin domain to drive peptide substrate preferences for the PGANT9 splice variants. These data highlight heretofore unknown mechanistic details governing substrate specificity that involves charge contributions from both the catalytic and lectin domains.
We also found that this splicing event influences glycopeptide preferences. Screening of a random glycopeptide library revealed differences between the preferred directionality of addition by each splice variant. Taken together, these results indicate that PGANT9A and PGANT9B have distinct glycopeptide preferences which vary among different glycopeptide substrates.
How the differentially spliced a repeats influence the recognition of extant GalNAc residues in different glycopeptide substrates remains to be investigated. Towards this goal, we are currently attempting to co-crystalize each splice variant with glycopeptide substrates to determine how extant GalNAcs are recognized by the lectin domain.
Our studies highlight splicing of enzyme subregions as another mechanism whereby the substrate preferences of the PGANT/GALNT family members may be fine-tuned based on the needs of the cells/tissues where they are expressed. This mechanism of altering substrate preferences could be crucial to ensuring proper glycosylation of diverse endogenous substrates.
For example, many mucins have charged residues within their serine and threonine-rich repeats while the repeat regions of other mucins are uncharged. Splicing that modulates whether a PGANT/GALNT will interact with a charged or uncharged mucin could be highly beneficial, especially in tissues where the mucin profile changes developmentally. As our current study demonstrates, charged residues in the catalytic domain as well as the lectin domain have the potential to influence substrate preferences. As lectin and catalytic subdomains lie in exonic regions of other members of this family (1), it suggests that splicing events that modify these domains may be used more widely by other PGANTs/GALNTs. In fact, we and others have detected putative splice variants for other PGANT and GALNT family members (39,40). For example, splicing of the human GALNT13 alters both the length and charged residues within the g repeat of the lectin domain (39,40). This splicing event results in changes in the affinities for certain glycopeptide substrates, but does not appear to alter directionality of GalNAc addition to glycopeptides (39,40). Whether splicing alters specific peptide preferences remains to be examined in more detail. Other mammalian splice variants have been predicted in silico (e.g. Galnt7, Galnt14, Galnt15, Galnt17, Galnt18), however whether splicing occurs in vivo and how it might alter peptide and/or glycopeptide preferences remains to be determined.
In summary, we have found a new regulatory paradigm whereby transferase activity/substrate preferences can be modified in vivo via splicing of specific subdomains within the lectin region. Additionally, we have further characterized how substrate preferences are influenced by a combination of charged residues in both the lectin domain and the flexible catalytic gating loop. Moreover, we have discovered that alteration of the lectin domain influences both peptide and glycopeptide preferences.
This discovery illustrates a new mechanism for modulating substrate preferences in vivo to accommodate the glycosylation demands of specific cells/tissues and highlights multi-faceted roles for the lectin domain in substrate recognition.

Experimental procedures Enzyme assays
Expression of recombinant PGANT9A and PGANT9B was performed either using Pichia pastoris or COS7 cells as described previously (21,38). Peptide substrates were synthesized by Peptide 2.0.
Assays for each peptide substrate were run in triplicate and repeated three times. Experimental values for each substrate were then averaged and standard deviations were calculated. Enzyme activity (initial rate) is expressed as DPM/min*µM.

PGANT9A and PGANT9B Activity Against Substrates With Flanking Charged Residues
A series of 9 differently charged peptide substrates designed to address the roles of flanking charged residues clusters (see Supplemental Table-S1) were custom synthesized by RS Synthesis (Loiusville, KY). The invariant sequences used in these model substrates contain the +1 and +3 preference for proline seen across most Oglycoslytransferases that contain a proline pocket in the catalytic domain (including PGANT9A and PGANT9B). The additional central invariant sequences are based on prior work defining the human GALNT3 (a related ortholog of PGANT9A and PGANT9B) optimal sequence -YAVTPGP- (20), which was flanked by all possible combinations of the three residues clusters; RRR, DDD and GAG (representing positive (basic, B), negative (acidic, A) and neutral control (N) clusters respectively). These were then further flanked by GAGA-and -AGAG sequences at the N-and Cterminal, respectively, resulting in a total of 9 different peptide substrates.
Identical transferase reactions were performed at the same time with PGANT9A and PGANT9B against 9 different charged substrates.
Reactions were initiated by placing 13 µl of a premade buffer mix containing acceptor, cofactor, detergent and enzyme (90 µl media in a total of 155 µl) into Eppendorf tubes containing 7 µl of each peptide substrate (1 mM). The reactions were capped and incubated for 4 hr in a shaking microincubator at 35 o C and quenched by the addition of two volumes of 50 mM EDTA (40 µl) on ice. The reaction time was optimized for less than 15 % product formation, and less than 50 % donor (UDP-[ 3 H]-GalNAc) utilization. Three independent experiments were performed with each set of peptide substrates for both PGANT9A and PGANT9B.

Characterization of Lectin Domain Specificity From Random Glycopeptide Substrates
The lectin domain probing random glycopeptide substrates GP(T*22)R and GP(T*10)L (and their non-glycosylated controls GP(A22)R and GP(A10)L) were utilized to compare the remote GalNAc-O-Thr glycopeptide specificity of PGANT9A and PGANT9B as previously described (17,25). (Note these glycopeptides were previously named GPIV, GPV, GPIV-C and GPV-C respectively, and synthesized by Sussex Research, Ottawa CA (17). Reactions were carried out using 68 mM sodium cacodylate, pH 6.5, 1.     Peptides based on the preferred single site acceptor, AT8, were designed to determine whether proline is important for activity (C). There was no activity detected for PGANT9A with these peptides. D. Peptides used in each reaction are shown. Error bars are SD. Each set of assays was repeated three times.
Statistical comparisons were performed between the first peptide shown and each other variant using the Student's t-test to calculate p values (**, p<0.01).