Diabetes enhances translation of Cd40 mRNA in murine retinal Müller glia via a 4E-BP1/2 – dependent mechanism

Activation of the immune costimulatory molecule cluster of differentiation 40 (CD40) in Müller glia has been implicated in the initiation of diabetes-induced retinal inflammation. Results from previous studies support that CD40 protein expression is elevated in Müller glia of diabetic mice; however, the mechanisms responsible for this increase have not been explored. Here, we evaluated the hypothesis that diabetes augments translation of the Cd40 mRNA. Mice receiving thiamet G (TMG), an inhibitor of the O -GlcNAc hydrolase O -GlcNAcase, exhibited enhanced retinal protein O -GlcNAcylation and increased Cd40 mRNA translation. TMG administration also promoted Cd40 mRNA association with Müller cell – specific ribosomes isolated from the retina of RiboTag mice. Similar effects on O -GlcNAcy-lation and Cd40 mRNA translation were also observed in the retina of a mouse model of type 1 diabetes. In cultured cells, TMG promoted sequestration of the cap-binding protein eIF4E (eukaryotic translation in initiation factor 4E) by 4E-BP1 (eIF4E-binding protein 1) and enhanced cap-independent Cd40 mRNA translation as assessed by a bicistronic reporter that con-tained the 5 9 -UTR of the Cd40 mRNA. Ablation of 4E-BP1/2 prevented the increase in Cd40 mRNA translation in TMG-exposed cells, and expression of a 4E-BP1 variant that constitutively sequesters eIF4E promoted reporter activity. Extending on the cell culture results, we found that in contrast to WT mice, diabetic 4E-BP1/2 – deficient mice did not exhibit enhanced retinal Cd40 mRNA translation and failed to up-regulate expression of the inflammatory marker nitric-oxide synthase 2. These findings support a model wherein diabetes-induced O -GlcNAcy-lation of 4E-BP1 promotes Cd40 mRNA translation in Müller glia.

Activation of the immune costimulatory molecule cluster of differentiation 40 (CD40) in Müller glia has been implicated in the initiation of diabetes-induced retinal inflammation. Results from previous studies support that CD40 protein expression is elevated in Müller glia of diabetic mice; however, the mechanisms responsible for this increase have not been explored. Here, we evaluated the hypothesis that diabetes augments translation of the Cd40 mRNA. Mice receiving thiamet G (TMG), an inhibitor of the O-GlcNAc hydrolase O-GlcNAcase, exhibited enhanced retinal protein O-GlcNAcylation and increased Cd40 mRNA translation. TMG administration also promoted Cd40 mRNA association with Müller cell-specific ribosomes isolated from the retina of RiboTag mice. Similar effects on O-GlcNAcylation and Cd40 mRNA translation were also observed in the retina of a mouse model of type 1 diabetes. In cultured cells, TMG promoted sequestration of the cap-binding protein eIF4E (eukaryotic translation in initiation factor 4E) by 4E-BP1 (eIF4E-binding protein 1) and enhanced cap-independent Cd40 mRNA translation as assessed by a bicistronic reporter that contained the 59-UTR of the Cd40 mRNA. Ablation of 4E-BP1/2 prevented the increase in Cd40 mRNA translation in TMGexposed cells, and expression of a 4E-BP1 variant that constitutively sequesters eIF4E promoted reporter activity. Extending on the cell culture results, we found that in contrast to WT mice, diabetic 4E-BP1/2-deficient mice did not exhibit enhanced retinal Cd40 mRNA translation and failed to up-regulate expression of the inflammatory marker nitric-oxide synthase 2. These findings support a model wherein diabetes-induced O-GlcNAcylation of 4E-BP1 promotes Cd40 mRNA translation in Müller glia.
Retinal inflammation plays a critical role in the pathogenesis of diabetic retinopathy (DR) (1,2). Although inflammation is typically beneficial when resolved promptly, chronic low-grade inflammation can contribute to disease progression (3). Diabetes increases the expression of proinflammatory molecules in retinal microglia, pericytes, endothelial cells, and Müller cells (3). The conventional view of retinal inflammation is that microglia are the first responders that initiate Müller cell activation and gliosis (4). Indeed, Müller cells respond to activated microglia by increasing the expression of proinflammatory factors including interleukins 1b and 6 (5). Counter to this conventional view, Portillo et al. (6) recently demonstrated that the immune costimulatory molecule cluster of differentiation 40 (CD40) in Müller glia is specifically responsible for initiating diabetes-induced retinal inflammation. Activation of CD40 by its ligand CD154 results in increased protein nitration, retinal leukostasis, and capillary degeneration, which are all hallmark characteristics of DR (7,8). Diabetic CD40 knockout mice fail to exhibit enhanced proinflammatory cytokine expression and are protected from both retinal leukostasis and degeneration of the retinal vasculature (6,8). Remarkably, Müller cell-targeted add-back of CD40 to whole body CD40 knockout mice restores the retinal inflammatory response to diabetes, as well as the development of leukostasis and capillary degeneration (6).
Post-translational modification of proteins by O-linked addition of b-D-GlcNAc (O-GlcNAcylation) regulates the inflammatory response under both physiological and pathological conditions (9). Elevated retinal protein O-GlcNAcylation has been reported in rodent models of both type 1 and type 2 diabetes, and evidence supports a role in the pathogenesis of DR (10,11). Protein O-GlcNAcylation occurs via the addition of the O-GlcNAc donor UDP-GlcNAc to the hydroxyl side chain of serine and threonine residues. Enzymatic addition of the O-GlcNAc moiety is catalyzed by O-GlcNAc transferase, whereas the modification is removed by the hydrolase O-GlcNAcase. Deficiency in protein O-GlcNAcylation inhibits T-cell proliferation and differentiation (12,13). Similarly, O-GlcNAcylation is necessary for B-cell expansion and activation of neutrophils (14,15). Several immune-stimulating transcription factors are O-GlcNAc modified, including NF-kB, NFAT, and SP1, resulting in enhanced transcriptional activity of these proteins and subsequent production of immunomodulatory molecules (16)(17)(18)(19)(20).
In the retina of diabetic mice, CD40 protein expression is elevated in Müller glia (6); however, the mechanism responsible for this increase has not been established. Expression of proinflammatory proteins is typically induced by transcription factors, which can promote inflammation by increasing the expression of cytokines and other proinflammatory molecules. Importantly, translational control also plays a critical role in the inflammatory response that is less well-explored (21). Despite evidence demonstrating widespread modification of ribosomal and other regulatory proteins, including the translational repressor 4E-BP1 (22)(23)(24)(25)(26) the level of mRNA translation (27). In that study, the top canonical pathway identified as being translationally altered by enhanced O-GlcNAcylation was the "acute-phase response," which is a complex early-defense system involving a range of proinflammatory cytokines. Here, we explored Cd40 as one gene candidate subject to translational regulation in retina.
The rate at which an mRNA is translated is generally limited by the recruitment of ribosomes, which can occur through either a cap-dependent or cap-independent mechanism. All eukaryotic mRNAs contain a 59-m 7 GTP cap structure that is recognized by the cap-binding protein eIF4E (28). Cap-dependent recruitment of a ribosome onto an mRNA occurs via assembly of the eIF4F complex (that includes eIF4E) at the 59-cap, followed by binding of eIF4F·mRNA to the 40S ribosomal subunit (28). Sequestration of eIF4E by the translational repressor 4E-BP1 prevents eIF4F complex assembly and represents the bestcharacterized mechanism for repressing cap-dependent translation initiation. As an alternative to cap-dependent translation, ;10% of mRNAs contain sequence elements that enable translation to be initiated independent of the 59-cap (29). These sequence elements are not conserved between genes; however, a common feature is the formation of hairpin, or stem-loop, secondary structures. Highly structured elements in the 59-UTR of messages are thought to enable translation under physiological duress when cap-dependent translation is inhibited, such as during exposure to diabetes-induced hyperglycemia (30,31). A number of cell surface receptors are subject to translational regulation and variation in receptor expression often reflects changes in downstream signaling (32). In this study, we evaluated the hypothesis that diabetes enhances translation of the Cd40 mRNA. Overall, the findings demonstrate that protein O-GlcNAcylation promotes cap-independent Cd40 mRNA translation via a 4E-BP1/2-dependent mechanism that involves sequences within its 59-UTR.

O-GlcNAcase inhibition enhances Cd40 mRNA translation in retina
We previously evaluated the impact of O-GlcNAcase inhibition on retinal gene expression using next-generation sequencing to assess changes in mRNA abundance and ribosomebound mRNAs undergoing translation via RNA-Seq and ribosome profiling (i.e. Ribo-Seq), respectively (27). Cd40 was identified as a candidate gene that exhibited enhanced mRNA translation in the retina of mice administered TMG as compared with PBS vehicle alone (Fig. 1A). As with the previous report (27), protein O-GlcNAcylation was enhanced in the retina of mice administered TMG as compared with PBS (Fig. 1B). Consistent with the results of ribosome profiling, Cd40 mRNA abundance was not altered in whole retinal lysates from mice administered TMG as compared with PBS (Fig. 1C). Sucrose density gradient centrifugation was used to separate retinal ribosomes into either heavy or light fractions (Fig. 1D). Poorly translated mRNAs are found in the light subpolysomal fraction, which contains both 80S monosomes, as well as individual 40S and 60S ribosomal subunits. In contrast, actively translating mRNAs associate with multiple ribosomes and form heavy pol-ysomes. Consistent with sequencing results in Fig. 1A, the Cd40 mRNA was shifted into the heavy fraction obtained from the retina of mice administered TMG, as compared with PBS ( Fig. 1E). In addition to up-regulation of Cd40 mRNA translation, mRNAs predicted by the sequencing analysis to exhibit down-regulation (Nfe2l2 and Lhx1) or no change (Nqo1) were also distributed in polysome profiles in a way that supported those observations (Fig. 1F).

Diabetes enhances Cd40 mRNA translation in retina
To assess whether Cd40 mRNA translation was enhanced in a model of diabetes, we generated polysome profiles from the retina of streptozotocin (STZ)-induced diabetic mice, a model of type 1 diabetes. Consistent with previous studies (11), blood glucose concentrations were significantly elevated ( Fig. 2A) and retinal protein O-GlcNAcylation was enhanced in diabetic mice as compared with nondiabetic controls (Fig. 2B). To determine whether diabetes altered Cd40 transcription, we evaluated Cd40 mRNA abundance. There was no significant change in Cd40 mRNA abundance in the retina of diabetic mice as compared with nondiabetic mice (Fig. 2C). However, the relative distribution of the Cd40 mRNA was shifted into the heavy fraction obtained from the retina of diabetic mice, indicating enhanced translation (Fig. 2D).

Diabetes and O-GlcNAcase inhibition enhance CD40 mRNA translation in retinal Müller glia
To specifically assess Cd40 mRNA translation in retinal Müller cells, we developed a RiboTag mouse (33) that expressed an epitope-tagged ribosomal protein under the control of Cre recombinase that is directed specifically toward retinal Müller cells by promoter/enhancer elements of the Pdgfra gene (34). RiboTag mice express a fully functional hemagglutinin (HA)tagged variant of ribosomal protein L22 (Rpl22), which forms the core of the ribosomal 60S subunit and facilitates cell typespecific isolation of translationally active mRNAs (33). Hemizygous PDGFRa-Cre-positive mice were crossed with RiboTag mice, resulting in deletion of WT exon 4 in Müller cells and replacement with an exon 4 that included an HA tag (Fig. 3A). Replacement of WT exon 4 with the HA-tagged exon 4 was validated by PCR (Fig. 3B). HA-tagged Rpl22 expression was only observed in the retina of Cre-positive mice (Fig. 3C). HA-Rpl22 expression colocalized with the Müller cell-specific marker glutamine synthetase (GS) in retinal cryosections (Fig.  3D). Müller-specific HA-tagged ribosomes were immunoprecipitated from whole retinal lysates (Fig. 3E). HA-Rpl22 was detected in immunoprecipitations targeting the HA tag but not in a FLAG-tag negative control. RNA isolated by immunoprecipitation was high quality (Fig. 3F). The immunoprecipitation efficiently isolated retinal mRNAs encoding Müller cellspecific markers (CRALBP, GS, and AQP4), but not the retinal pigmented epithelium marker RPE65 (Fig. 3G). To evaluate the effect of enhanced O-GlcNAcylation on Cd40 mRNA translation, RiboTag mice were administered TMG. Cd40 mRNA association with HA-tagged ribosomes was enhanced in the retina of mice administered TMG as compared with those administered PBS, indicating up-regulated Cd40 mRNA translation (Fig. 3H). Similarly, STZ-induced diabetes also promoted Cd40 mRNA translation in retinal Müller cells (Fig. 3I).

Role of 4E-BP1/2 in cap-independent Cd40 mRNA translation
Transcript-specific translational control is generally mediated by cis-regulatory elements encoded by the UTR of the target mRNA. A recent genome-wide systematic screen indicated that sequences within the 59-UTR of the Cd40 mRNA were capable of promoting translation independent of a 59 mRNA cap (35). In addition, we previously provided evidence that enhanced O-GlcNAcylation causes a shift from cap-dependent to cap-independent mRNA translation that is mediated by 4E-BP1 (31). To investigate the impact of 4E-BP1 on Cd40 mRNA translation, we established a cell culture model of enhanced O-GlcNAcylation in the presence and absence of 4E-BP1. In both WT and 4E-BP1/2-deficient cells, exposure to TMG enhanced protein O-GlcNAcylation (Fig. 4A). TMG promoted 4E-BP1 coimmunoprecipitation with eIF4E, whereas the protein could not be detected in 4E-BP1/2-deficient cells (Fig. 4, B and C). Mfold web server predicted that the 59-UTR sequence of Cd40 forms a hairpin structure (Fig. 4D). To specifically evaluate the role of the 59-UTR of Cd40, it was cloned into a bicistronic reporter (pYIC) wherein a single mRNA encodes both yellow and cyan fluorescent protein (YFP and CFP, respectively). YFP expression was used as a reporter for cap-dependent translation, whereas CFP expression was used as a reporter for cap-independent translation driven by the Cd40 59-UTR (Fig. 4E). In WT cells, exposure to TMG increased the CFP/YFP expression ratio, whereas there was no change in the ratio of CFP/YFP expression in 4E-BP1/2-deficient cells exposed to TMG (Fig.  4F). We also coexpressed pYIC with a 4E-BP1 F113A variant that resists mTORC1-dependent phosphorylation and therefore constitutively binds eIF4E (36) in the human MIO-M1  Müller cell line. As compared with an empty vector, 4E-BP1 F113A increased the CFP/YFP expression ratio (Fig. 4G). These data support a role for 4E-BP1 in promoting Cd40 mRNA translation.

4E-BP1/2 deletion prevents diabetes-induced CD40 mRNA translation
To evaluate the role of 4E-BP1 in regulating the translation of the Cd40 mRNA in the retina, diabetes was induced in WT and 4E-BP1/2 knockout mice. Blood glucose concentrations (Fig. 5A) and retinal protein O-GlcNAcylation (Fig. 5B) were significantly elevated in both WT and 4E-BP1/2-deficient diabetic mice as compared with nondiabetic mice. No significant change in total Cd40 mRNA was detected in whole retinal lysates (Fig. 5C). In polysome profiles, the Cd40 mRNA shifted into the heavy fraction obtained from the retina of WT diabetic mice as compared with WT nondiabetic mice (Fig. 5D). However, the distribution of the Cd40 mRNA in the heavy fraction obtained from the retina of 4E-BP1/2-deficient diabetic mice was similar to that observed in 4E-BP1/2-deficient nondiabetic mice (Fig. 5D). Consistent with changes in mRNA translation, CD40 protein expression was increased in the retina of WT STZ-diabetic mice, and 4E-BP1/2 deletion prevented the effect (Fig. 5E). Because CD40 is necessary for the retinal inflammatory response to diabetes, inducible nitric-oxide synthase 2 (Nos2) mRNA abundance was evaluated in retinal lysates as a marker for retinal inflammation. Diabetes enhanced Nos2 expression in the retina of WT mice; however, there was no difference in Nos2 expression in the retina of diabetic and nondiabetic 4E-BP1/2-deficient mice (Fig. 5F).

Discussion
The TNF receptor superfamily member CD40 is a central mediator of inflammation in DR pathology (6,8,37). CD40 protein expression in Müller glia is elevated in the retina of diabetic rodents and is critical for the development of retinal inflammation in diabetes (6). In the present study, we demonstrated that in the retina of STZ-diabetic mice, CD40 protein expression was enhanced, independent of a change in total Cd40 mRNA abundance. Rather, the increase in CD40 protein expression in the retina of diabetic mice was associated with increased translation of the Cd40 mRNA. Using a novel RiboTag mouse model, we provide evidence that STZ-induced diabetes enhanced Cd40 mRNA translation in retinal Müller glia. In the retina of diabetic rats and in retinal Müller glia exposed to hyperglycemic conditions, the sequestration of eIF4E by 4E-BP1 is enhanced (38,39). Our laboratory previously demonstrated that 4E-BP1 is directly O-GlcNAc-modified in response to diabetes (26,40) and that O-GlcNAcylation promotes binding of 4E-BP1 with eIF4E (26,31). Herein, the effect of diabetes on Cd40 mRNA translation required eIF4E sequestration, because 4E-BP1/2 ablation prevented diabetes-induced Cd40 mRNA translation in retina and normalized expression of CD40 protein, as well as the inflammatory marker NOS2. Retinal NOS2 expression is enhanced in both patients with DR and in rodent models of the disease (41,42). Up-regulated expression of this proinflammatory marker is critical for disease progression, because NOS2-deficient mice are resistant to diabetes-induced retinal leukostasis and capillary degeneration (43,44). Overall, the results here are consistent with a model wherein diabetes-induced O-GlcNAcylation facilitates retinal inflammation by promoting Cd40 mRNA translation (Fig. S1). An important caveat to this model is that there is likely a myriad of other signaling changes that promote the retinal inflammatory response in diabetes. Thus, it will be important for future studies to further interrogate both CD40-dependent and CD40-independent signaling events that contribute to retinal pathology in diabetes.
Portillo et al. (6)(7)(8) provided evidence for a model wherein Müller glia initiate retinal inflammation via CD40 activation, with eIF4E in B was quantified. D, a hairpin structure was predicted for the Cd40 59-UTR using the mfold web server. E, a bicistronic reporter was generated wherein a single mRNA encodes for both YFP and CFP. YFP expression is mediated by cap-dependent translation, whereas cap-independent expression of CFP is under the control of the Cd40 59-UTR. F, WT and 4E-BP1/2 double knockout (4EBP-DKO) mouse embryonic fibroblasts expressing Cd40 reporter were cultured in the presence of TMG or vehicle for 24 h. G, human MIO-M1 Müller cell cultures were cotransfected with the Cd40 reporter, and either a plasmid that expresses an empty vector (EV) or a 4E-BP1 F113A variant that constitutively binds eIF4E. CFP, YFP, tubulin, and 4E-BP1 expression were assessed by Western blotting of cell lysates. The ratio of CFP to YFP expression was quantified from Western blots. The values are means 6 S.D. A Student's t test was used to compare differences between groups in C and G. The data in E were analyzed by two-way ANOVA (Table S1). Dunnett's post hoc analysis was used to identify differences between means when significant main and interaction effects were detected. *, p , 0.05 versus vehicle; #, p , 0.05 versus WT.
Diabetes promotes Cd40 mRNA translation which leads to ATP release and stimulation of P2X7 purinergic receptors on microglia/macrophages. In the retina of diabetic CD40 knockout mice, NOS2 expression, leukostasis, and the development of acellular capillaries are reduced as compared with diabetic WT control (6,8). However, Müller cell-targeted add-back of CD40 to CD40 knockout mice restores diabetesinduced retinal inflammation as assessed by inflammatory cytokine expression (e.g. NOS2) (6). Despite the in vivo work pinpointing Müller cells as a critical mediator of CD40-driven inflammation in DR, in vitro work revealed that both human and rodent Müller glia are unable to secrete cytokines in response to CD40 ligation by its ligand CD154 (6). This raised the possibility that CD40 signals to neighboring microglial cells to stimulate the production of proinflammatory cytokines. Indeed, Portillo et al. (6) found that activation of CD40 on Müller glia induced proinflammatory responses through signaling to bystander microglia. Overall, the previous findings support a model in which CD40 activation on Müller glia initiates the inflammatory response in DR. It is worth noting that other groups have not independently verified such a model. Moreover, the studies here were not designed to do so.
Herein, we explored a potential mechanism responsible for increased CD40 expression in retinal Müller glia of diabetic mice. We investigated translational regulation of the Cd40 mRNA, based on recent genome-wide screens that supported the possibility (27,35). In the retina of diabetic mice, we found that CD40 mRNA translation was enhanced in Müller glia concomitant with an increase in retinal protein O-GlcNAcylation. In addition, Cd40 mRNA translation was enhanced in Müller glia of mice administered TMG to promote retinal protein O-GlcNAcylation.
Activation of CD40 on retinal Müller glia depends upon the presence of its ligand CD154. CD154 concentrations are enhanced in the blood of both diabetic patients and rodents, and plasma levels of soluble CD154 are positively correlated with the severity of DR (45,46). It remains unclear how CD154 gets into the retina prior to the development of inflammation; however, it is possible that microthrombosis in the retinal capillaries of patients with DR enables activation of platelets in the retina (47). Activated platelets express surface CD154 that is rapidly shed upon ligation with CD40-expressing endothelial cells into the biologically active soluble CD154 (48). Moreover, ligation of CD154-expressing platelets with CD40-expressing endothelial cells stimulates inflammatory cascades (45). Alternatively, in advanced stages of DR when immune privilege and the blood-retinal barrier are compromised, circulating immune cells (such as CD154-expressing T cells) or serum proteins (such as soluble CD154) may infiltrate the retina and contribute to inflammation-induced neurovascular retinal damage (49).  (Table  S1). Dunnett's post hoc analysis was used to identify differences between means when significant main or interaction effects were detected. *, p , 0.05 versus vehicle; #, p , 0.05 versus WT.
In response to diabetes, Müller cells exhibit a range of protein expression changes that contribute to retinal inflammation, microvascular defects, and neuronal dysfunction (reviewed by Coughlin et al. in Ref. 50). Müller cells are uniquely sensitive to the metabolic environment in diabetes, in part because of their exclusive expression of the highcapacity/low-affinity GLUT2 glucose transporter (51) and predominance of the feedback inhibition-resistant glutamine-fructose-6-phosphate amidotransferase 2 isoform (52). Importantly, glutamine-fructose-6-phosphate amidotransferase is the rate-limiting enzyme of the hexosamine biosynthetic pathway. Thus, hyperglycemia potentially has dramatic effects on protein O-GlcNAcylation in Müller cells of diabetic patients. Diabetes causes Müller cells to become reactive and exhibit increased expression of the intermediate filament protein glial fibrillary acidic protein (53)(54)(55). In the retina of diabetic patients, increased glial fibrillary acidic protein expression manifests prior to development of DR, suggesting an early onset of Müller glia dysfunction (56)(57)(58).
CD40 expression is generally low under basal conditions, and increased CD40 expression is a common feature of inflammatory diseases driven by the receptor (59). A previous report supports that CD40 protein expression is elevated in Müller glia of diabetic rodents (6). Previous studies also demonstrate that TNFa and IFN-g from microglia activate Cd40 gene transcription via cis-elements in the Cd40 promoter (60). However, cytokine-mediated Cd40 transcriptional activation is not consistent with a model wherein CD40 expression in Müller glia initiates retinal inflammation (6). Rather, transcriptional activation of CD40 through this conventional pathway would represent a model wherein retinal inflammation is initiated by gene expression changes in the microglia. In the present study, we provide new evidence that diabetes promotes Cd40 mRNA translation independent of a change in total Cd40 mRNA abundance.
We previously reported that augmented O-GlcNAcylation contributes to retinal dysfunction by causing widespread variation in the selection of mRNAs for translation, as assessed by ribosome profiling (27). CD40 was identified in that next-generation sequencing analysis as a gene candidate in which expression in retina was primarily altered at the level of mRNA translation. Translational control of CD40 expression is supported by a previous study demonstrating that a Graves' disease-associated single nucleotide polymorphism in the Cd40 59-UTR results in increased Cd40 mRNA translation and protein expression (61). As previously mentioned, Cd40 was also identified in a genome-wide screen for sequence elements capable of promoting cap-independent translation (35). In the present study we cloned the 59-UTR of the Cd40 mRNA into a reporter construct to evaluate cap-independent Cd40 mRNA translation. The 59-UTR of Cd40 mRNA is not notably long at only 77 base pairs but is particularly GC rich (72%) and predicted to form a stable stem-loop structure. It is worth noting that this sequence also contains a consensus G-quadruplex (59-. . .GGGCGGGGCCAAGGCTGGGGCAGGG. . .) (62). When located within the 59-UTR, GC-rich secondary structures such as these inhibit cap-dependent mRNA translation (63,64). However, these same highly structured RNA elements often facilitate preferential translation when cap-dependent translation is inhibited (30,31). In the present study, we found that retinal Cd40 mRNA translation was enhanced by exposure to TMG. This is consistent with a relative increase in the Cd40 59-UTR reporter that was observed in cells exposed to TMG. Importantly, TMG failed to enhance the relative activity of the Cd40 59-UTR in the absence of 4E-BP1/2, whereas the 4E-BP1 F113A variant, which cannot be phosphorylated, was sufficient to enhance translation from the reporter. Collectively, our observations support a role for TMG-induced translational control of CD40 expression that is mediated by the Cd40 59-UTR and requires 4E-BP1/2. Despite significant efforts, we were not able to consistently evaluate translation of endogenous Cd40 mRNA in polysomes prepared from MEFs. This is at least in part because of low Cd40 mRNA abundance in the cell culture model.
In the present study, 4-week-old mice were administered either TMG to promote O-GlcNAcylation or the toxin STZ to model type 1 diabetes. Retinal polysomes were prepared 24 h after TMG administration or 3 months after the induction of diabetes. Importantly, retinal Cd40 mRNA translation was increased by both TMG and STZ relative to their respective controls. The Cd40 mRNA was almost exclusively observed in the light subpolysomal fraction of 4-week-old mice 24 h after administration of a vehicle control. By contrast, CD40 was more evenly distributed between the light and heavy fractions obtained from the retina of nondiabetic control mice. These observations suggest that Cd40 mRNA translation may be increased in the retina of older mice. They also raise the possibility that factors other than O-GlcNAcylation potentially influence Cd40 mRNA translation.
Anti-inflammatory steroids are used clinically to treat diabetic macular edema, and the implantation of the corticosteroid dexamethasone provides a therapeutic alternative for diabetic macular edema patients refractory to the first-line anti-vascular endothelial growth factor therapies (65). However, long-acting dexamethasone implants are associated with an increased risk for elevations in intraocular pressure and cataract formation (65). Therefore, there is a need to identify alternative therapies for patients who do not respond to anti-vascular endothelial growth factor treatment. The previous body of work indicates that CD40 activation specifically on Müller glia drives the expression of multiple cytokines and chemokines, resulting in augmented inflammatory signaling and ultimately chronic retinal inflammation associated with DR. We extend the previous studies by demonstrating that diabetes and enhanced O-GlcNAcylation promote Cd40 mRNA translation via a 4E-BP1/2-dependent mechanism. Based on the data here, 4E-BP1/2-mediated control of CD40 expression in Müller glia may represent a therapeutic target for intervention to prevent retinal inflammation in DR. Notably, suppression of O-GlcNAc transferase activity by angiotensin-converting enzyme inhibition represents a potential mechanism for combating the increase in 4E-BP1 O-GlcNAcylation that is seen in diabetes (66). Regardless, further mechanistic studies delineating CD40mediated retinal inflammation are needed to better understand the role of CD40 activation in Müller glia in DR.

Experimental procedures
Animals At 4 weeks of age, male and female C57BL/6 mice received 50 mg/kg TMG or PBS as a control via intraperitoneal injection. The retinas were extracted 24 h after TMG injections. Alternatively, male WT or 4E-BP1/2 (Eif4ebp1; Eif4ebp2) double knockout mice were administered 50 mg/kg streptozotocin via intraperitoneal injection for 5 consecutive days to induce diabetes. Control mice received equivalent volumes of sodium citrate buffer. Diabetes was confirmed by blood glucose concentration (.250 mg/dl) in fasted animals. The retinas were extracted after 4 or 12 weeks of diabetes. All procedures were approved by the Pennsylvania State College of Medicine Institutional Animal Care and Use Committee and in accordance with the Association for Research in Vision and Ophthalmology policies on the ethical use of animals in ophthalmic research.

Protein analysis
The retinas were extracted, flash-frozen in liquid nitrogen, and later homogenized in 250 ml of extraction buffer as described previously (38). The homogenate was centrifuged at 1000 3 g for 5 min at 4°C, and the supernatant was collected for analysis. A fraction of the supernatant was added to SDS sample buffer, boiled for 5 min, and analyzed by Western blotting analysis as described previously (67). Antibodies used included O-GlcNAc (CTD110.6) (Cell Signaling Technology, catalog no. 9875), CD40 (Cell Signaling Technology, catalog no. 86165), a-tubulin (Santa Cruz, catalog no. sc-32293), GAPDH (Santa Cruz, catalog no. sc-32233), HA (Santa Cruz, catalog no. sc-805), and DDK (Origene, catalog no. TA5011-100). Preparation of the eIF4E, 4E-BP1, and eIF4G antibodies has been described previously (68,69). Immunoprecipitations were performed by incubating cell lysates with monoclonal anti-eIF4E antibody as described previously (31).

Polysome fractionation by sucrose density gradient centrifugation and RNA isolation
Sucrose density gradient centrifugation was employed to separate the subpolysomal from the polysomal ribosome fractions as described previously (27). Two sucrose fractions representing the light and heavy portions of the gradient were collected directly into an equal volume of TRIzol reagent (Invitrogen). To improve the recovery of RNA from dense sucrose portions of the gradient, the heavy fraction was diluted twice with RNase-free water (HyClone), and the appropriate amount of TRIzol was added. RNA was extracted using the standard manufacturer's protocol and resuspended in 14 ml of RNA storage solution. An equal amount of RNA from each fraction collected was converted into cDNA using a highcapacity cDNA reverse transcription kit (Applied Biosystems) and subjected to quantitative real-time PCR using QuantiTect SYBR Green Master Mix (Qiagen).

RiboTag mouse model
B6J.129(Cg)-Rpl22 tm1.1Psam /SjJ (RiboTag) mice carrying a targeted homozygous mutation of the ribosomal protein L22 locus to enable Cre-mediated HA epitope tagging of ribosomes from specific cell types were purchased from The Jackson Laboratory. To enable expression of HA-tagged ribosomes in Müller glia, RiboTag mice were crossed with hemizygous C57Bl/6-Tg(Pdgfra-cre)1Clc/J (PDGFRa-CRE; The Jackson Laboratory) to generate heterozygous RiboTag mice. The resulting heterozygous generation was crossed with homozygous RiboTag mice to produce offspring with a PDGFRa-cre recombinase-expressing mouse with deletion of WT exon 4 of the Rpl22 locus and replacement with the Rpl22 HA exon specifically in Müller cells of the retina.

Isolation of Müller-specific ribosomes
Ribosomes were immunoprecipitated from RiboTag mice as previously described in Ref. 33. Briefly, EZview anti-HA beads (Sigma) were washed twice in immunoprecipitation buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl 2 , 1% Nonidet P-40) and blocked with 0.5% BSA, followed by two additional washes in immunoprecipitation buffer before being added to retinal homogenates. Two retinas from a single mouse were homogenized in polysome buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl 2 , 1% Nonidet P-40, 1 mM DTT, 400 units/ml Promega RNasin, 0.5 mg/ml heparin, 100 mg/ml cycloheximide, 10 ml/ml protease inhibitor mixture (Sigma) followed by centrifugation at 10,000 3 g for 10 min at 4°C. Supernatants were added to prepared beads and allowed to rotate for 16 h at 4°C. The beads were pelleted at 8200 3 g for 30 s, the supernatant was removed, and the beads were washed thrice with high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl 2 , 1% Nonidet P-40, 1 mM DTT, 100 mg/ml cycloheximide). For Western blotting analysis, 4% of the beads and 2% of the input, and supernatant were placed in Laemmli buffer. Total RNA was processed by adding 375 ml of RLT buffer (Qiagen) to the remaining beads and following manufacturer's protocol using an RNeasy Micro kit (Qiagen). RNA quantity was assessed with the NanoDrop Lite spectrophotometer (Thermo Scientific), whereas RNA quality was assessed with the RNA 2100 bioanalyzer (Agilent).

Bicistronic reporter assay
A dsDNA gBlock fragment (IDT) encoding the 59-UTR of Cd40 (59-TTTCCTGGGCGGGGCCAAGGCTGGGGCAGG-GGAGTCAGCAGAGGCCTCGCTCGGGCGCCCAGTGGT-CCTGCCGCCTGGTCTCACCTCGCTATG-39) was cloned into pCR2.1TOPO using the TOPO TA cloning kit (Invitrogen) according to the manufacturer's instructions. pYIC and TOPO plasmids were digested with EcoRI-HF (New England BioLabs) and BamHI-HF (New England BioLabs). A 4700-base pair fragment and 100-base pair fragment were gel-purified from pYICand TOPO-digested plasmids, respectively. The fragments were ligated and cloned into XL-1 blue supercompetent cells (Agilent). The resulting construct was digested with BstX1 (New England BioLabs) to remove the ECMV IRES. The digested construct was gel-purified, ligated, and cloned into XL-1 blue supercompetent cells. The reporter was sequenceverified using the primer ATCACATGGTCCTGCTGG.

Statistical analysis
All statistical analysis was performed with GraphPad Prism (Version 8.2.0). A Student's t test was used to compare differences between groups. In Figs. 4E and 5, where appropriate, the data were analyzed with two-way ANOVA with Dunnett's post hoc analysis (Table S1). The data are expressed as means 6 S. D. Significance was defined as p , 0.05 for all analyses.

Data availability
All data for this publication are included in the article and supporting information or are available from the corresponding author upon request.