Bacterial iron detoxification at the molecular level

Iron is an essential micronutrient, and, in the case of bacteria, its availability is commonly a growth-limiting factor. However, correct functioning of cells requires that the labile pool of chelatable “free” iron be tightly regulated. Correct metalation of proteins requiring iron as a cofactor demands that such a readily accessible source of iron exist, but overaccumulation results in an oxidative burden that, if unchecked, would lead to cell death. The toxicity of iron stems from its potential to catalyze formation of reactive oxygen species that, in addition to causing damage to biological molecules, can also lead to the formation of reactive nitrogen species. To avoid iron-mediated oxidative stress, bacteria utilize iron-dependent global regulators to sense the iron status of the cell and regulate the expression of proteins involved in the acquisition, storage, and efflux of iron accordingly. Here, we survey the current understanding of the structure and mechanism of the important members of each of these classes of protein. Diversity in the details of iron homeostasis mechanisms reflect the differing nutritional stresses resulting from the wide variety of ecological niches that bacteria inhabit. However, in this review, we seek to highlight the similarities of iron homeostasis between different bacteria, while acknowledging important variations. In this way, we hope to illustrate how bacteria have evolved common approaches to overcome the dual problems of the insolubility and potential toxicity of iron.

A great deal of the biological importance of iron stems from facile redox transformations between the Fe 21 and Fe 31 oxidation states that underpin its function as a cofactor in many enzymes. Iron-containing proteins are grouped into three main classes. Iron-sulfur clusters are thought to represent the oldest class of iron-containing cofactors. They typically consist of 2-4 iron ions (although occasionally more) but occasionally also contain a heterometal, such as nickel or molybdenum, linked by inorganic sulfide and covalently attached to the protein via the thiol groups of cysteine residues. These versatile cofactors are involved in many processes, including respiration, photosynthesis, nitrogen fixation, hydrogen evolution, and the associated electron transfer chains (1). The simplest iron-containing cofactors are formed by the binding of discrete metal ion to sites composed from the side chains of histidine and/or the carboxylates aspartate and glutamate. These are principally employed to harness the oxidizing power of O 2 for processes such as DNA synthesis and methane oxidation (2). Heme is formed by the incorporation of iron into the tetrapyrrole protoporphyrin IX. This chemically versatile cofactor is critical in many processes, including respiration, cycling of nitrogen, and sulfur and detoxification reactions in addition to also supporting electron transfer (3)(4)(5). As a result of this versatility, the demand for iron is large in most organisms, including the majority of bacteria, with up to 25% of the proteome binding iron in some form (6).
However, the same redox chemistry required for these roles (Reaction 1 and the Fenton reaction, Reaction 2) allows iron to catalyze the Haber-Weiss reaction (Reaction 3).
Fe 31  The resulting hydroxyl radicals ( OH) are highly reactive, causing damage to lipids, proteins, carbohydrates, and nucleic acids (7). Superoxide (O 2 2 ) and hydrogen peroxide (H 2 O 2 ) are produced as by-products of aerobic respiration (8), and, therefore, any aerobically respiring organism faces the requirement not only to detoxify ROS but also to strictly regulate the concentration of iron in any form able to catalyze the Haber-Weiss reaction. This need is particularly acute in the case of bacteria because, in addition to endogenously produced ROS, they are often subjected to assault by ROS produced either by competitors in the environment or in phagocytes produced by the immune system of hosts during infection (9).
Nitric oxide is known to play an important role as a signaling molecule in biological systems but is also produced in elevated concentrations for defense or competition in a similar manner to ROS. Combination of nitric oxide with superoxide generates the peroxynitrite ion that is susceptible to further oxidation to either nitrogen dioxide or dinitrogen trioxide. Collectively, these RNS can cause damage to nucleic acids and modify the side chains of amino acids such that protein structure and function are impaired (9). Furthermore, both ROS and RNS are known to lead to breakdown of iron-sulfur clusters, resulting in the displacement of iron from the cofactor. Thus, iron homeostasis and the generation of ROS and RNS are intimately connected, as are the regulatory networks for their management within bacterial cells.

Sensing of iron and regulation of genes involved in iron uptake/homeostasis
When considering the iron status of cells, it is important to distinguish between the quota, which is the total iron content of the cell, and that subset of the quota that is kinetically available for insertion into proteins and molecular cofactors, referred to as the "labile iron pool" (10). The majority of the latter is likely in the Fe 21 oxidation state and coordinated by small molecules, such as low-molecular weight thiols (11,12). This represents the fraction of the quota available to fulfill metabolic requirement, but also that with the potential to catalyze unwanted ROS and RNS formation. Therefore, the first requirement of any regulatory system for iron homeostasis is the ability to sense the concentration of the labile iron pool across the physiologically relevant range, 1-10 mM according to most estimates (13)(14)(15). As one might expect, this is achieved by transcriptional regulators whose affinities for target DNA are modulated by either binding directly to iron or by the binding of iron-dependent prosthetic groups. Often these are global regulators, controlling the expression of a great many genes, including those involved in the biosynthesis and import of siderophores, import of ferrous iron, and the storage and/or efflux of iron present in excess of cellular requirements. This balancing of metal trafficking to fulfill nutritional requirements while suppressing potential toxicity, shown schematically in Fig. 1, is termed "nutritional passivation" and is a common strategy that extends beyond iron metabolism (16).

Iron sensing by Fur
Members of the Fur (ferric uptake regulator) superfamily are the most widespread transcriptional regulators controlling iron homeostasis in bacteria. The first member of the Fur family was identified in Escherichia coli some 35 years ago (17) and, as the name suggests, was reported to regulate the intake of Fe 31 into the cell. This is achieved by the binding of the protein to "Fur boxes," AT-rich binding sites upstream of the regulated genes with the consensus sequence 59-GATAATGATAATCAT-TATC- 39. It has been argued that the Fur box should be considered a 21-bp fragment containing two overlapping 7-1-7 inverted repeats that each bind a Fur dimer.

Scheme 1.
These are positioned such that the two copies of Fur bind to opposite faces of the DNA helix (18). Binding of Fur occludes access of RNA polymerase, thus repressing transcription of the responsive genes (19). However, despite the great deal of research effort directed at members of the Fur superfamily, an understanding of these processes at the molecular level has only recently been achieved.
Despite reports of both monomeric (20) and higher oligomeric (21) forms of Fur detected in solution, the physiologically relevant form of the protein is thought to be the homodimer. This is stabilized by a large buried interface between C-terminal dimerization domains (22) and, in most cases, the binding of a structural Zn 21 (23) ion by four conserved Cys residues (24). Occupancy of this structural site (S1) is required, but not Heavy arrows depict intracellular movement of iron, light arrows show the movement of iron or ironbearing compounds across the cell membrane, and lines connect the transcriptional regulators to systems under their control. When the concentration of the labile iron pool increases, iron, or an iron-containing group, binds to the transcriptional regulator. This leads to down-regulation of processes such as siderophore synthesis, export of apo-siderophores, import of Fe 31 -siderophores, heme import, and Fe 21 uptake systems. Simultaneously, expression of iron-containing and iron storage proteins is up-regulated together, occasionally, with iron efflux pumps. Reduction in the labile iron pool leads to dissociation of iron/ iron-containing groups from the regulators, resulting in the opposite transcriptional responses. sufficient, for DNA binding. The Fur family exhibits some structural variation, and in certain examples, the dimerization domain harbors a second structural site ligated by His and Glu residues (25). The dimerization domain is connected to the Nterminal DNA-binding domain via a flexible hinge region containing a regulatory site comprising His and Glu side chains that binds Fe 21 with a reported dissociation constant, K d , of ;1 mM when determined in vitro (26). Whereas the regulatory site has been demonstrated to bind other di-and trivalent metals, it is thought that only Fe 21 is present at the concentration required to activate the protein in vivo. Occupancy of this site induces a rotation of the DNA-binding domain relative to the dimerization domain, creating an increased void area between the two DNA-binding domains such that they are able to accommodate dsDNA (25). It is thought that this conformational change forms the molecular basis of the increased affinity of Fur for DNA in vitro under elevated concentrations of the regulatory metal. In vitro studies utilizing gel-shift methods report K d values of ;10 nM for complex formation between activated Fur and target DNA sequences (23).
The recently reported crystal structure of Magnetospirillum gryphiswaldense Fur (27) in complex with DNA has provided insight into the molecular basis for recognition of Fur-binding sites (Fig. 2). The AT-rich composition of the Fur box results in a narrowing of the minor groove and consequent increase in negative charge density from the phosphate backbone that persists upon repressor binding. This facilitates shape recognition by Fur via a favorable electrostatic interaction between a conserved lysine residue (Lys-15 in M. gryphiswaldense Fur numbering) and the minor groove. More specific interactions with bases in the major groove are facilitated by the rotation of the DNA-binding domains induced by metal binding at the regulatory site. This involves van der Waals interactions between Tyr-56 and consecutive thymine bases in the target sequences and hydrogen bonding between the guanidinium group of Arg-57 and the O6 and N7 atoms of a conserved guanine. A recent report suggests that Fur DNA binding can be tuned by proteinprotein interactions (28), in addition to the long-recognized effect of iron binding. EIIA Ntr , a component of the nitrogen metabolic phosphotransferase system, was shown to affect expression of Fur-regulated genes. In vitro gel shift measure-ments showed that this arises from formation of a protein-protein complex that lowers the affinity of holo-Fur for DNA. Consequently, repression of Fur-regulated genes requires a greater cytoplasmic Fe 21 concentration when EIIA Ntr is present. The K d for the Fur-EIIA Ntr complex has not yet been determined; nor has the increase in K d of the Fur-DNA complex in the presence of EIIA Ntr .
In addition to the classic gene repression mechanism described above, Fur has been shown to act as an activator of gene expression, both directly (29-32) and indirectly (33). Direct activation occurs through binding in the promoter region (29)(30)(31)(32), whereas indirect regulation occurs via interaction with the noncoding RNA RhyB (see the Iron storage in bacteria section below) (34), by the displacement of histone-like proteins (35), or by blocking the binding of a second repressor (36). Regulation of gene expression by apo-Fur has also been demonstrated in a limited number of cases (37,38), and genome-wide studies have demonstrated the Fur regulon to encompass dozens of transcription units, containing .100 genes in some cases (33,37,(39)(40)(41). It is now apparent that Fur-like proteins constitute a superfamily with members identified that are responsive to other metals (Mur, the manganese uptake regulator (42), and Zur (43), the zinc uptake regulator) and to peroxide-induced oxidative stress (Per) (23).
Genes identified as being regulated by Fur, such as that in E. coli, include those encoding iron-uptake systems, such as fhu, fec, and feo; the suf iron-sulfur cluster assembly system; iron-sulfur-containing proteins, such as fumA, acnA, acnB, and nuo; the iron-containing superoxide dismutase sodB; and the iron storage proteins bfr and ftnA (see below). Consistent with its role as a repressor of iron import systems, the transcriptional response of a Fur deletion mutant is similar to that evoked by iron limitation, even under iron-replete conditions. This inability to correctly sense the iron status of the cell has been demonstrated to result in an increase in ROS production (44), suggesting that, in contrast to some other metals, cellular storage and efflux mechanisms are unable to compensate for the resulting elevated concentration of the labile iron pool. Fur has been shown to be involved in the remodeling of cell metabolism away from iron-containing enzymes, management of ROS, and reconfiguration of the cell membrane to protect against antibiotic attack, in addition to controlling cellular iron homeostasis (10,33,45).

Iron sensing by DtxR/IdeR
Proteins of the DtxR/IdeR (diphtheria toxin repressor/irondependent regulator) family are the global transcriptional regulators controlling iron uptake in GC-rich Gram-positive bacteria (46). Indeed, DtxR was first identified as an iron-dependent repressor of virulence factor expression in Corynebacterium diphtheriae, and it is from this activity that the name derives (47). Much effort has been devoted to the study of this group of bacteria as they include important human pathogens such as C. diphtheriae itself, Mycobacterium tuberculosis, and Staphylococcus aureus and antibiotic producers such as Streptomyces. This included the demonstration that DtxR also regulates iron uptake in C. diphtheriae and the identification of homologues in other organisms.
Proteins of this family exhibit similarities to Fur; they act primarily as repressors of transcription by occluding binding of RNA polymerase (48,49) but recognize a consensus sequence with greater GC content than that of Fur: 59-TTA-GGTTAGCCTAACCTAA-39 (50). The homodimers harbor multiple metal-binding sites and undergo conformational change upon binding Fe 21 as corepressor. In the metalbound active form, dsDNA binds between two helix-turnhelix (HTH) N-terminal DNA-binding domains that are linked via dimerization domains (51,52). In vitro DNA affinity of Fe 21 -sensing DtxR proteins is also activated by noncognate divalent metal ions such as Ni 21 , Co 21 , Mn 21 , and Cd 21 . Ni 21 and Fe 21 bind DtxR with the highest affinity, K d being around 1 mM (53, 54). However, distinct from Fur, these proteins also contain an SH3-like domain of unknown function as a C-terminal extension (52). They also differ in the molecular contacts leading to recognition of target DNA and the nature of the conformational change induced by binding of the regulatory metal.
Structures of DtxR in complex with DNA were available before those of Fur and revealed two homodimers bound to each nucleotide fragment (51) (Fig. 3). Each of the monomers harbors two metal-binding sites (presumed to be iron in vivo), and, in further analogy to Fur, binding of divalent metal to the high-affinity ancillary site imparts stability to the protein fold, whereas affinity for target DNA sequences is increased by the occupancy of the lower-affinity primary site (46,55,56). However, in contrast to Fur, occupancy of the primary metal-binding site results in only a small rotation of the DNA-binding domains relative to the dimerization domains (52). Comparison of apo-and holo-structures of DtxR suggests that metal ion binding induces a helix-to-coil transition in the six N-terminal residues (51,56) and relieves what would, in the apoprotein, be an unfavorable steric interaction with DNA. This, together with a small "caliper-like" movement of the N-terminal domains, which brings them into better alignment with the major groove, results in the increased DNA affinity for the holo form of the repressor over the apo form (51). Residues 27-50 make up the helixturn-helix DNA-binding motif containing helices B and C. Each monomer contributes a total of nine favorable interactions with nucleotide phosphate groups: Arg-27, Ala-28, and Arg-29 of helix B and Thr-40, Ser-42, Arg-47, and Arg-50 of helix C together with Glu-36 and Ser-37 of the intervening loop. In further contrast to Fur, formation of the protein-DNA complex causes distortion of the nucleotide from the B-form conformation. This results in the opening up of the minor groove such that it can accommodate the guanidinium group of Arg-60, located on the loop 57-61 connecting two strands of an antiparallel b-sheet (51).
Examples of the DtxR/IdeR family lacking the C-terminal SH3-like domain have been reported, but these are not responsive to Fe 21 in vivo (57,58). Given the recent discovery that DNA binding by Fur is modulated by formation of a complex with EIIA Ntr , it is possible that the SH3 domain modulates the iron response of DtxR via protein-protein interactions. The suite of genes regulated by DtxR includes those involved in siderophore production and translocation, heme degradation, Fe 21 import, iron-sulfur cluster assembly, and iron storage (59), demonstrating similar regulatory activity to Fur despite there being no evolutionary link between the two protein families.

Iron sensing by RirA and Irr
The genomes of the a-proteobacteria contain homologues of Fur, but where these have been characterized, they have been shown either to have a diminished role in iron regulation compared with other examples of Fur or to be responsive to other metals, such as Mn 21 (60,61). Global regulation of iron is performed by two novel transcriptional regulators found, with few exceptions, only within the a proteobacteria (62): iron response regulator (Irr) (63) and rhizobial iron regulator A (RirA) (64). These are currently less well-characterized than either Fur or DtxR, with no crystal structures of either the proteins or protein/DNA complexes available to date. However, significant progress in understanding these proteins has been made recently, and both are known to sense the availability of intracellular iron not by binding the metal itself, but instead by binding iron-containing prosthetic groups.
RirA is unique among bacterial iron-sensing transcriptional regulators in that it belongs to the Rrf2 family (64). As with many members of this family, affinity of RirA for DNA is modulated by the binding of an iron-sulfur cluster (65). Again, the protein exists as a homodimer in solution, and homology modeling based on the recently reported structures of other Rrf2 regulators predicts that each monomer contains a DNA-binding domain, featuring a winged helix-turn-helix motif, connected to a dimerization helix via a loop containing three conserved Cys residues (66).
Under iron-replete conditions, the protein contains a [4Fe-4S] 21 cluster, coordinated by the three conserved Cys residues (67) (with a likely additional, but unknown, ligand), and binds to cis-acting iron-responsive operator sequences (68) (IRO boxes) in the promoter region of genes involved in iron uptake acting as a repressor of transcription in a manner analogous to Fur and DtxR. The apoprotein lacks any specific high-affinity interaction with DNA in vitro, whereas a meta-stable [2Fe-2S] 21 cluster-containing form has been shown to exhibit intermediate binding affinity (69). RirA has also been shown to promote transcription of genes (70,71), including those involved in iron storage under iron-replete conditions, via an indirect mechanism involving small noncoding RNA (72) 11 form and is subsequently mediated by the oxidation of cluster sulfides. This susceptibility to O 2 -mediated iron and sulfur oxidation is thought to underpin a dual Fe 21 -and O 2 -sensing role. RirA has been demonstrated to regulate iron-sulfur cluster biogenesis in R. leguminosarum, and O 2 sensing by RirA may be important to ensure adequate cellular supply of iron-sulfur clusters under aerobic conditions even when iron is replete. An as yet unknown regulatory mechanism prevents up-regulation of iron-uptake systems under these conditions (66).
Whereas RirA is restricted to the order rhizobiales, Irr is widely distributed among the a-proteobacteria (72,73). The protein is a homologue of Fur but senses the iron status of the cell not by binding Fe 21 from the free iron pool, but the ironcontaining prosthetic group heme (43). Due to the insolubility and potential cytotoxicity of heme, cells are unlikely to contain a "free heme pool" akin to that of Fe 21 . Rather, it is thought that Irr is associated with ferrochelatase (74), the enzyme responsible for insertion of iron into protoporphyrin IX in the final step of heme biogenesis, and acquires the prosthetic group directly from it. Apo-Irr binds to iron control element (67,75) sequences (ICE boxes) that are upstream of regulated genes and, like other Fur proteins, can act directly either as a repressor or an activator, depending on the location of the ICE sequence (75). However, in the case of Irr, direct activation of regulated genes is far more common than for either Fur or DtxR. In further contrast to other examples of the Fur superfamily, Irr only binds to ICE sequences in the absence of its coregulator. All examples characterized to date contain two heme-binding sites. One of these is a conserved HXH motif (76,77), but studies have revealed significant diversity in the nature of the other. Possibly related to this, the mechanism by which derepression occurs appears to differ markedly between members of the rhizobiales in which RirA also acts as an iron-responsive global regulator and other a-proteobacteria in which Irr is the only protein fulfilling this function. The best-characterized examples are the Irr proteins from R. leguminosarum (belonging to the former class) and Bradyrhizobium japonicum (from the latter).
In organisms such as R. leguminosarum, Agrobacterium tumefaciens (78), and Ensifer meliloti (72), Irr forms part of a regulatory network involving RirA among other factors. These networks are interlinked, with Irr controlling expression of RirA while the two proteins regulate iron homeostasis in an antiparallel manner. Under high-iron conditions, RirA represses the expression of iron uptake systems, whereas in low iron Irr represses the expression of iron storage systems but also RirA, thereby assisting in derepression of RirA-regulated genes. The proteins are dimeric in solution, and loss of DNA-binding affinity is associated with the binding of heme at the HXH motif located close to the interface between the monomers (77). Disruption of this heme-binding motif by mutagenesis led not only to the abolition of heme binding but also high-affinity binding of DNA by the apoprotein, thereby demonstrating the importance of this motif for the recognition of ICE box sequences. These observations led to a model in which a conformational change in the HXH motif upon binding of heme forms the molecular basis of the loss of DNA affinity. However, the detail of any such conformational change at the atomic level remains to be elucidated. Whereas the regulatory role, if any, of the second heme-binding site remains unclear, its occupancy has been shown to modulate the oligomeric state of the protein in vitro (79).
In organisms such as B. japonicum, in which Irr is the only global regulator of iron homeostasis, regulation is achieved via a different mechanism. These proteins have an HXH hemebinding motif similar to that identified in Irr from Rhizobiales (76,80), but this site preferentially binds heme with iron in the Fe 21 oxidation state. Furthermore, the binding of heme does not affect the affinity of the protein for DNA binding; rather, the protein has been shown to be conditionally stable with degradation initiated by the binding of ferric heme at a second site, the heme regulatory motif. On binding to this site, the heme iron is five-coordinate with the sulfur of a cysteine residue providing the axial ligand. Pulsed radiolysis studies demonstrated a ligand switch to axial ligation by histidine upon reduction followed by binding of O 2 under aerobic conditions (81). This has led to the suggestion of a ROS-mediated pathway for B. japonicum Irr degradation in the presence of heme. The available data indicate that heme binding to both sites of B. japonicum Irr is required for efficient degradation of the protein. The heme regulatory motif is not limited to B. japonicum Irr, having also been identified in Irr proteins from Nitrobacter, Xanthobacter, and Magnetospirrilum (61), suggesting a similar mechanism of iron regulation in these organisms.
In the absence of both of its substrates, ferrochelatase binds Irr with high affinity, thereby competing with DNA binding and alleviating regulatory activity. However, binding of protoporphyrin IX to ferrochelatase causes dissociation of its complex with Irr. Therefore, when the rate of heme synthesis outstrips the availability of iron, Irr is released, down-regulating iron-dependent biosynthetic pathways and activating genes involved in iron acquisition. Once the concentration of Fe 21 in the labile iron pool increases sufficiently such that metalation of protoporphyrin IX is coordinated with its synthesis, heme is inserted into Irr, targeting the protein for oxidative degradation and therefore ensuring that regulatory activity is abrogated. It is thought that this system of regulation allows the rate of iron uptake to be matched to metabolic need under varying conditions rather than simply maintaining the labile iron pool at a concentration determined by the affinity of Fe 21 for the transcriptional regulator (74).

Iron acquisition by bacteria
Despite its natural abundance in the earth's crust, iron is often a growth-limiting micronutrient for bacteria due to the insolubility of the Fe 31 ion at neutral pH, which limits the dissolved iron concentration to 1.4 3 10 29 M under aerobic conditions (82). To counter the low bioavailability of iron in many environments, bacteria have evolved high-affinity iron acquisition pathways. Whereas these are often targeted by host immune systems or competing bacteria to limit growth (83), they are also under the control of the global regulators described above to enable expression to be repressed should iron availability exceed cellular requirement (84). Iron uptake in bacteria has been extensively studied with the ultimate aim of preventing infection by targeting iron metabolism. Here we survey the main features while referring the interested reader to several recent reviews (85-88).

Siderophore-mediated iron uptake
The most widely distributed iron acquisition strategy under aerobic conditions is the secretion of siderophores (89). These are small-molecule chelators  Da) (90) with high affinity for Fe 31 (K d in the range 10 220 to 10 249 M) that acquire iron from the extracellular environment (85). Over 500 examples have been characterized to date falling in to three main classes, the catechols, hydroxamates, and a-hydroxycarboxylates, defined according to the nature of the iron-ligating moiety (89). Examples containing more than one of the aforementioned iron-ligating groups are termed mixed siderophores.
Siderophore synthesis is nonribosomal but occurs in the cytoplasm, meaning that in Gram-negative bacteria, their export and, in most cases, utilization of the sequestered iron requires transport across both the cytoplasmic and periplasmic membranes. There appears to be the greatest diversity in the proteins involved in the export across the cytoplasmic membrane, with examples belonging to both the ABC transporter (91) and major facilitator superfamily (MFS) (92) classes reported. Export across the outer membrane is mediated by TolC-like efflux pumps (93).
Once secreted from the cell, siderophores acquire iron either by outcompeting host proteins, such as transferrin, or by the solubilization of Fe 31 from iron-containing minerals. Import across the outer membrane is mediated by porins (Fig. 4A) composed of 22-stranded b-barrels and an extracellular facing "plug" domain that binds the iron-loaded siderophore with high (typically nanomolar) affinity. The TonB/ExbBD energytransducing complex spans the periplasmic space and connects the porin to the cytoplasmic membrane potential, allowing active transport of the substrate.
Once internalized, periplasmic binding proteins (Fig. 4B) act as chaperones delivering the ferric siderophore complex to the cytoplasmic membrane. Here ABC transporters (Fig. 4D) couple transport across the inner membrane to ATP hydrolysis. Many bacteria are able to utilize multiple siderophores to satisfy their requirement for iron, including "xenosiderophores," those that the organism is unable to synthesize but can internalize and extract iron from (94). A general trend is that the outer-membrane porins show specificity for their cognate siderophore, whereas the inner-membrane ABC transporters have greater flexibility in substrates tolerated. Therefore, the genomes of Gram-negative bacteria encode a greater number of outer-membrane porins for siderophore uptake than ABC transporters dedicated to the same task (95).
Once the loaded siderophore has been translocated to the cytoplasm, the iron is typically released via reduction to Fe 21 (95), for which the chelators have lower affinity. A possible exception to this are the hexadentate triscatechelates, which form the most stable Fe 31 complexes of all siderophores, stabilizing this oxidation state to such an extent that the midpoint of the Fe 31 /Fe 21 couple is in the range 2600 to 2750 mV (versus SHE). It is thought that esterase-mediated hydrolysis of the backbone, resulting in three bidentate catechol units, is required for iron release. This raises the midpoint potential of the chelated iron to around 2350 mV, which is accessible to intracellular reductants, such as NADH (E m ; 2320 mV) (85,95). Other exceptions to the scheme outlined above are known, most notably for the pyoverdines, the principle siderophores of some pseudomonads, where reductive iron release occurs in the periplasm (96).

Extraction of iron from heme
In the case of many pathogenic bacteria, heme represents an important source of iron because it accounts for some 75% of the iron content of mammals (97). The heme acquisition pathway shows many parallels to siderophore uptake, perhaps reflecting the insolubility and potential toxicity of both heme and Fe 31 .
In some cases, such as the Has system of Pseudomonas aeruginosa, heme scavenging proteins termed hemophores are secreted to the extracellular environment (98). These proteins ligate heme via the side chains of conserved His and Tyr residues (99,100). In contrast to siderophores, they deliver the extracted heme to outer-membrane heme-binding proteins and are not themselves reimported to the cell. The outer-membrane proteins bind heme via two histidine residues and have a lower intrinsic affinity for heme than hemophores. However, formation of the hemophore/outer-membrane binding protein complex induces a conformational change in the hemophore, lowering its affinity for heme and ensuring transfer in the desired direction (101).
In other systems, such as Phu also from P. aeruginosa, the outer-membrane receptors acquire heme directly from host proteins (102). Whereas PhuR, the outer-membrane hemebinding protein of Phu, employs His/Tyr ligation of heme (103), it appears that His/His ligation is more common among these proteins (86). In either case, they bind heme with picomolar affinity and are able to extract it from host proteins such as hemoglobin or the hemoglobin-haptoglobin complex (86).
The outer-membrane heme-binding proteins are associated with 22-25-stranded b-barrel porins (Fig. 4A). These are also coupled to the cytoplasmic membrane potential by the TonB/ ExbBD complex. As with siderophores, heme is shuttled to the inner membrane by periplasmic binding proteins (Fig. 4C) and imported to the cytoplasm by ABC transporters (Fig. 4D) (86). Once located in the cytoplasm, heme can be directly incorporated into bacterial proteins, but is more commonly subjected to oxidative degradation by heme oxygenases to liberate the iron (104). Heme acquisition systems are subject to negative regulation by the iron-dependent transcriptional regulators to avoid iron overload, but expression is also linked to sensing of heme availability by hemophores via extracytoplasmic function s factors (105).

Uptake of ferrous iron
Under acidic and/or anaerobic conditions, iron is predominantly in the soluble ferrous oxidation state. Consequently, bacteria have evolved mechanisms for the direct uptake of iron in this form. The solubility of Fe 21 means that active transport across the outer membrane of Gram-negative bacteria is not required, and it enters the periplasm by free diffusion through porins (106). Several systems have been demonstrated to import Fe 21 into the cytoplasm, including MntH (107), ZupT (108), YfeABCD (109), FutABC (110), EfeUOB (111), and Feo, but of these, only Feo appears both widespread and dedicated to the transport of Fe 21 (106).
Feo was first identified in E. coli, where the operon encodes three proteins, FeoA, FeoB, and FeoC (112). However, it seems that FeoC is limited to the g-proteobacteria (88), and the most commonly occurring (54% of sequenced genomes) feo gene organization consists of only feoAB, whereas 11% of sequenced bacterial genomes contain feoB alone (106). FeoB is an ;80-kDa membrane protein containing 7-12 transmembrane helices (106). A cytoplasmic domain located at the N terminus has been shown to bind and hydrolyze GTP (113)(114)(115), with hydrolysis thought to be activated by K 1 (116). At present, it is unclear whether this supports active transport of the Fe 21 substrate or is used to signal the energy status of the cell. This GTPase domain is linked to the membrane-spanning helices by a GDP dissociation inhibitor domain (117) and switch regions thought to alter conformation upon nucleotide binding. The mechanism by which FeoB transports Fe 21 remains elusive but is thought to be mediated by binding of the metal to the sulfur atoms of Cys and Met residues located in the transmembrane helices (106).
Both feoA and feoC encode small (;8-kDa) hydrophilic proteins. FeoA is a basic protein with pI at around pH 9.0, consistent with localization to the inner leaf of the cytoplasmic membrane (118). The protein displays significant homology to SH3 domains and possess the same fold (119). This has led to the suggestion that protein-protein interactions between FeoA and the GTPase domain of FeoB regulate the rate of nucleotide hydrolysis. Whereas deletion of feoA has been shown to result in a 60% reduction in Fe 21 transport (88), direct interaction between FeoA and FeoB has not yet been demonstrated. FeoC adopts the winged helix-turn-helix fold (120, 121) common in DNA-binding domains and from its structure has been predicted to be a repressor of transcription (118,122). However, DNA-binding activity of FeoC remains to be demonstrated.
The Feo system exemplifies the complex interplay of iron and O 2 metabolism that is likely a universal characteristic of bacteria. Under anaerobic conditions, the expression of ferric import systems decreases due to an increase of Fe 21 -Fur. The feo operon is also negatively regulated by Fur, thereby preventing iron overload. However, at typical intracellular iron concentrations, the combined positive regulation of feo by ArcA and FNR alleviates Fur-mediated repression (15). In this way, anaerobic conditions lead to the repression of ferric iron uptake systems, whereas the expression of feo, the importer matched to the most likely available iron source, has been reported to increase 3-fold under anaerobic conditions (88).

Iron uptake in Gram-positive bacteria
The iron acquisition pathways of Gram-positive bacteria show significant similarity to the Gram-negative systems described above despite the absence of an outer membrane and periplasmic space. Both siderophore-bound iron and heme are transported across the cell membrane by ABC transporters, whereas the Feo system is employed for the import of ferrous iron (87,98). Iron is also extracted from internalized heme by heme oxygenase enzymes (123,124).
Clearly, there is no requirement for either outer-membrane porins or periplasmic binding proteins. However, heme is unable to diffuse across the 15-80 nm of the peptidoglycan cell wall. Transport of heme across the cell wall is mediated by a series of proteins anchored at the cell surface. The Isd heme uptake pathway of Staphylococcus aureus is the most extensively studied of the Gram-positive systems and is thought to be representative of the general mechanism these bacteria employ for heme uptake (87). Four proteins are required for the transfer of heme across the cell wall to the IsdE/F ABC transporter complex. These are anchored to the cell surface by the sortases SrtA and SrtB (125,126). In each of the four surfaceanchored proteins, heme is bound at NEAT (near iron transporter) domains containing conserved YXXXXY domains in which the leading Tyr serves as a ligand to the heme iron (127). IsdB and IsdH extract heme from host proteins, whereas IsdA and IsdC shuttle the extracted heme to the ABC transporter complex with IsdC acting as the central conduit for transfer to IsdE/F (128). The unidirectional transfer of substrate is driven by the increasing affinity for heme of sequential NEAT domains in the shuttle pathway (129).
Tyrosine is an unusual heme ligand among heme-binding proteins in general but is prevalent among the proteins involved in bacterial heme acquisition. The hemophores and periplasmic binding proteins of the Gram-negative bacteria, in addition to those involved in transfer of heme across the cell wall in the Gram-positive case, all utilize tyrosine as a ligand, suggesting that its properties may be particularly suited to the capture and transfer of heme.

Iron storage in bacteria
Iron acquired via the mechanisms described above initially enters the labile iron pool. The existence of an intracellular pool of iron not bound to proteins was initially postulated on thermodynamic grounds (130). Because iron-utilizing proteins typically bind the metal with K d on the order 10 28 to 10 27 M, it was argued that a population of free metal with concentration greater than this must exist to prevent dissociation. This was presumed to be composed of Fe 21 as a result of the reducing environment of the cytoplasm and the requirement for rapid ligand exchange, the kinetic lability of Fe 21 complexes being typically 10 4 times greater than their Fe 31 counterparts.
Despite its critical importance in iron homeostasis, the chemical composition of the labile iron pool remains the source of considerable debate, in part due to the difficulty of defining the speciation of intracellular iron. Siderophores, amino acids, citrate, and low-molecular weight thiols have all been proposed as candidate ligands. Whole-cell Mössbauer spectroscopy provides the most direct empirical insight. The feature assigned to the labile iron pool has parameters typical of high-spin ferrous iron and is commonly interpreted as resulting from oxygen and nitrogen ligation (131). The relative affinities for Fe 21 and intracellular abundance of the proposed chelators makes citrate the most likely candidate of the oxygen donor ligands listed above. However, GSH (or equivalent low-molecular weight thiols such as mycothiol in the actinobacteria or bacillithiol in the firmicutes) is predicted to outcompete citrate at typical cytoplasmic concentrations and pH, leading to the counterproposal that the labile iron pool is dominated (up to 80%) by [Fe(H 2 O) 5 GSH] 21 or similar complexes (12). The prevalence of water in the coordination sphere of the Fe 21 would likely result in the high-spin electronic configuration reported by Mössbauer spectroscopy, despite the presence of a thiol ligand. Therefore, on balance, it seems likely that thiol-coordinated Fe 21 constitutes a major component of the labile iron pool.
Intriguingly, a very recent report suggests that polyphosphate acts as a hexadentate chelator of iron in vivo. Not only does this inorganic polymer act as a repressor of the Fenton reaction by saturating the coordination sphere of the metal; it has also been shown to act as an intracellular buffer of free iron (132). The extent to which this inorganic macromolecule contributes to either the labile iron pool or the long-term iron storage capacity of bacterial cells remains to be established.
The primary purpose of the labile iron pool is thought to be to ensure correct metalation of the iron proteome, which has been estimated to account for 60% of intracellular iron in cells grown on iron-replete (50 mM) liquid medium (133). However, as a result of the scarcity of iron, and despite the potentially catastrophic consequences of the Fenton reaction (Reaction 2), when the concentration of the labile iron pool exceeds this metabolic requirement, the excess is not simply excreted from the cell via efflux mechanisms. Rather, dedicated iron storage proteins belonging to the ferritin superfamily are employed to sequester the metal in a nonreactive state, which can be remobilized to satisfy cellular requirements during iron starvation. The signal pathway triggering the release of these iron stores remains to be elucidated, but it is reasonable to assume that the initial event would be depletion of the labile iron pool, leading to demetalation of iron-dependent transcriptional regulators.
These are composed of H-and L-chains, which, respectively, contain and lack a catalytic site for iron oxidation, but which are isostructural and can thus co-assemble in different proportions, depending on the organism/tissue. In contrast, bacterial genomes commonly encode multiple predicted ferritins of different classes. These include prokaryotic analogues of the animal ferritins called Ftns, heme-containing 24-meric ferritins, called Bfrs, that are unique to bacteria, and mini-ferritins, which are dodecamers that have only been identified in prokaryotes. All prokaryotic ferritin subunits contain a catalytic center for the oxidation of iron and assemble into homopolymers (134).
All ferritins share a four-a-helical bundle structural motif, and all except the L-chain units of animal cells contain di-iron catalytic sites, called ferroxidase centers, for the oxidation of iron (136) (Fig. 5). These are described in more detail below for each class of the bacterial proteins. Typical ferritins self-assemble into cagelike structures. The mini-ferritins form dodecamers of tetrahedral 3 3 2 symmetry with internal and external diameters of 4.5 and 9 nm, respectively (Fig. 5A), and possess additional helical elements at the N terminus and 2-fold axis (137). All other cage-forming ferritins possess only a short fifth helix (E) at the C terminus, altering the packing geometry. As a result, they assemble into larger rhombic dodecahedral cages possessing octahedral 4 3 2 symmetry with internal and external diameters of 8 and 12 nm, respectively (138) (Fig. 5C). All of these cagelike structures are permeated by channels at the vertices of their packing motifs that span the protein coat, connecting the interior cavity to bulk solution. The 4-fold channels of 24-meric ferritins are lined by the E helices, but variants in which this helix is missing are still competent to form assemblies with iron-storing capability. Rather, assembly of the protein is impaired by the disruption of residues at the C terminus of helix D (139).
The channels located at the 3-fold axes have been demonstrated to constitute the route of iron entry into animal ferritins (140). Comparatively little work has been reported on iron entry into the proteins from prokaryotes. Whereas some may also utilize the 3-fold channel (141), the so-called B-channels are used in at least a subset (142). These channels, which are found almost exclusively in prokaryotic ferritins, are formed at the 2-fold axis at the intersection between three monomeric units.
The proposal of dedicated routes for the transportation of Fe 21 from bulk solution through the protein coat to the site of oxidation has faced resistance due to the existence of a channel directly linking the ferroxidase center to bulk solution (143). However, there is increasing evidence that networks of carboxylate residues with conformational flexibility play key roles in Fe 21 transfer in all cage-forming ferritins (141,142,(144)(145)(146). All ferritins sequester Fe 21 from solution and utilize an electron-accepting co-substrate, such as O 2 or H 2 O 2 , to drive its oxidation to the Fe 31 state. This oxidized product is then translocated to the interior cavity, where it is stored as a hydrated ferric oxy mineral similar to ferrihydrite. Up to several thousand iron atoms per protein can be stored in this way. However, the molecular architecture of the catalytic centers carrying out this chemistry and the mechanistic detail of how it is achieved vary between the different classes of bacterial protein (147).
Expression of the mini-ferritins is usually regulated by s factors under nutritional stress or in response to oxidative stress (148), whereas that of the 24-meric examples is usually controlled by iron-responsive transcriptional regulators. However, unlike systems for iron uptake, this cannot be achieved by a mechanism of direct repression under high concentrations of free iron. For example, under low-iron conditions in E. coli, production of Ftn and Bfr proteins is repressed by the small RNA RyhB (34), which binds to ftn and bfr mRNAs (as well as many others), affecting translation through a number of mechanisms that include inhibiting translation and promoting mRNA degradation. RyhB is repressed by Fur so that, at elevated iron concentrations, the metalated protein down-regulates RyhB, leading to increased levels of Ftn and Bfr proteins. It has also been reported that expression of ftnA can be induced by Fur in a RhyB-independent manner (35). The mRNA-binding global regulator CsrA plays an important role in iron homeostasis, through its repression of genes such as bfr and dps (149), expression of which are not required under exponential, minimal stress conditions. In rhizobiales, bfr expression is directly repressed by Irr under iron limitation, with RirA implicated in derepression as iron availability increased (72). However, in some examples of cyanobacteria, iron storage is not positively regulated by increasing iron concentration (150,151). These observations further illustrate the complexity of cellular iron regulation.

Iron oxidation in Ftns
The Ftns are the closest analogues to the eukaryotic ferritins found in bacteria and are also widely distributed among archaea. The crystal structures of several examples are available, including that of the most intensively studied, FtnA of E. coli (138). These reveal an asymmetric di-iron ferroxidase center with similar architecture to that of the H-chain ferritins from animals (Fig. 5D). The predicted high-affinity site (site A) is coordinated by a bidentate Glu (17 in E. coli FtnA numbering), His-53, and bridging Glu-50 that also coordinates the predicted lower-affinity site (site B). Coordination of the second site is completed by monodentate Glu-94 and, in most examples, a second Glu (Glu-130 in E. coli). This residue also ligates a third metal-binding site (site C) whose coordination is completed by a further three monodentate Glu residues (Glu-49, -126, and -129). A conserved Tyr residue (Tyr-24) is also located close to site B and forms a hydrogen bond to one of the site B ligands .
In vitro studies of recombinantly expressed proteins have been employed to interrogate the mechanism by which Ftns lay down a mineral core within their interior cavity and have revealed marked similarity to that of their counterparts from eukaryotes. Under aerobic conditions, and in the absence of alternative co-substrate such as H 2 O 2 , O 2 binds to the freshly occupied di-Fe 21 center, resulting in the rapid formation of a di-Fe 31 -peroxo intermediate that is detectable via a transient absorbance feature in the wavelength range 600-650 nm (152). Hydrolysis of this intermediate results in the formation of a ferric-oxo species thought to be the precursor of the mineral core, which is not stably bound at the ferroxidase center (153). It remains to be demonstrated how the oxidized product is transported from the site of oxidation to the cavity, although this may involve the growth of iron-oxo clusters from carboxylate side chains located on the inner surface of the protein coat in close proximity to the ferroxidase centers.
The effect of substitutions of site C residues suggests that it is involved in ferroxidase center activity in some instances, although the roles of both site C and the conserved nearby tyrosine residue appear variable between different proteins (147). Some examples of Ftn exhibit a stoichiometry of their iron/oxygen chemistry that is greater than 2:1 and is affected, together with the rate of iron oxidation, by disruption of site C, suggesting a role for this site in Fe 21 oxidation/catalytic turnover. In others, the site appears to function to regulate the rate of flux of the oxidized product out of the ferroxidase center, such that flux is greater in the absence of site C. A role has been postulated for the conserved Tyr as a "molecular capacitor" providing, together with the three Fe 21 ions bound at sites A-C, four reducing equivalents enabling the direct reduction of O 2 to H 2 O (154). However, whereas every reported example of an Hchain like ferritin contains a Tyr residue at the equivalent position to Tyr-24 of E. coli FtnA, the effect of substitution of this residue (e.g. by Phe) is variable (147), suggesting that its function is variable. Furthermore, whereas some data support a role for conserved ferritin Tyr residues as electron donors, this is not always the case. In some instances, observation of di-Fe 31 peroxo species requires that assays be performed with a large excess of Fe 21 over ferroxidase center sites (155). These are precisely the conditions under which site C would be expected to be occupied and involvement of a third Fe 21 ion and oxidation of a Tyr residue would result in the direct formation of H 2 O. However, the observation of a di-Fe 31 peroxo species that decays to form the di-Fe 31 center and H 2 O 2 indicates that H 2 O is not formed and, therefore, that the conserved Tyr does not function as a reductant.
Regardless of the route of iron exit from Ftn ferroxidase centers, it is apparent that oxidized iron is translocated from here into the interior of the protein, regenerating empty binding sites, facilitating catalytic turnover. Furthermore, the rate of this flux is increased by further incoming Fe 21 substrate. This "displacement" model of core formation is directly analogous to that proposed for eukaryotic ferritins (153), although the effect of helix E deletion on the ability to generate a mineral core is different between the two classes of protein (139,156,157), which may reflect different routes of Fe 31 exit from the catalytic centers.

Iron oxidation in Bfrs
The most striking difference between the Ftns and Bfrs is the presence in the latter of 12 heme groups, located at the monomer-monomer interface of each of the subunit dimers that make up the 12 faces of the rhombic dodecahedral protein assembly. In vitro data indicate that the presence or absence of these prosthetic groups has little effect on the rate of iron uptake by the protein (158), particularly at low iron loadings. Instead, they are thought to promote the reductive mobilization of the mineral core (159) via their interaction with a small [2Fe-2S] cluster-containing ferredoxin, called Bfd (160), that is differentially expressed from bfr despite its adjacent location on many bacterial genomes.
The coordination of iron at the ferroxidase center also differs significantly between Bfr and Ftn. The catalytic center of the former is almost symmetric (Fig. 5D), with each metal ion coordinated by two bridging Glu residues (51 and 127, E. coli protein residue numbering), a His (54 at site A and 130 at site B), and a monodentate Glu (18 at site A and 94 at site B) (161). The E. coli protein remains the most extensively characterized example of Bfr, and, here at least, the difference in iron coordination at the ferroxidase center relative to other ferritins has an impact on the mechanism (although this is not the case for all; see below). Rather than releasing oxidized iron from the ferroxidase center into the interior of the protein, iron bound here appears to be a stable cofactor regardless of oxidation state (162), presumably as a consequence of the increased coordination number. Nevertheless, in vitro assays of iron mineralization activity demonstrate that the protein is able to lay down a mineral core containing up to 2800 eq of iron (163). Therefore, oxidized iron must be deposited in the interior of the protein via a route other than the displacement mechanism employed by the Ftns and other ferritins.
Crystallographic studies identified an iron-binding site, Fe IS , located on the inner surface of the protein that is important for function (161). This, together with a network of aromatic residues, including the tyrosine conserved in other classes of ferritin (Tyr-25 in this instance), deliver electrons into the ferroxidase center, generating Fe 31 within the protein cage in the process (164,165). The reduced ferroxidase center then reacts with a further oxidizing equivalent completing the catalytic cycle. Unlike the Ftns, the stoichiometry of the Fe/O 2 reaction is 4:1, consistent with H 2 O 2 being a far more effective co-substrate than O 2 for Bfr (166).
To a first approximation, the ligation of iron at the ferroxidase center of Pseudomonas aeruginosa Bfr (BfrB) 4 is identical to that in the E. coli protein. However, the structure of the protein derived from crystals subjected to different soaking conditions demonstrated conformational flexibility in residue His-130 (167). Whereas this residue acts as a ligand to iron in site B for structures in which the ferroxidase center is occupied, these sites are vacant in crystals formed from the protein as isolated, and His-130 in these structures is rotated relative to those with metal-containing active sites such that it would be unable to bond to a metal ion located at site B. These observations led to the proposal that the ferroxidase center of P. aeruginosa Bfr behaves as a gated pore for iron entry to the protein and a displacement mechanism of core formation akin to that of the Ftns. It is noteworthy that the rate at which the P. aeruginosa and E. coli Bfr proteins oxidize Fe 21 following binding of the metal to apo-ferroxidase centers is similar, but the former is able to lay down a mineral core at a rate far greater than the latter, consistent with mechanistic differences between them. The structure-function relationships governing these differences have not yet been resolved.

The roles of Ftn and Bfr vary between organisms
In E. coli, an ftnA deletion mutant exhibited marked impairment of growth compared with the WT strain on transfer from iron-replete to iron-deficient conditions (168). This phenotype was not observed for the bfr mutant, suggesting a role other than iron storage for this protein, possibly in oxidative stress response. In contrast, deletion of the bfrB gene in P. aeruginosa severely impairs the ability of the organism to accumulate iron as FtnA does not sequester a mineral core even in the absence of Bfr. Deletion of bfd or disruption of the Bfr/Bfd interaction elicits an iron starvation response, even under iron-replete conditions due to irreversible deposition of iron within the BfrB core (169). Therefore, it appears that the roles of Ftn and Bfr are reversed in the two organisms, and this may correlate with the reported differences in mineralization mechanism. A similarly variable picture is emerging from studies of ferritins in other organisms. For example, in Salmonella enterica, Bfr appears to be the major iron store (170), whereas, in M. tuberculosis, Ftn (previously known as BfrB 4 ) is important for virulence (171) and under high iron levels, whereas Bfr (BfrA) appears to be important for recycling iron under low iron levels (172). In the strictly anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris, Bfr plays an important role in protecting the organism from O 2 , which is normally toxic to such bacteria (173).

Iron oxidation by Dps/Dpr proteins
The Dps (DNA-binding proteins under starvation) proteins are composed of 12 identical a-helical subunits (rather than 24) and are consequently also known as mini-ferritins. They are significantly up-regulated during stationary phase or periods of oxidative stress (174). In addition to consuming the Fenton reagents Fe 21 and H 2 O 2 , they bind nonspecifically to DNA (175,176). This provides a physical barrier and can induce a crystalline transition in the nucleoid (177,178), both of which are thought to protect against oxidative damage. The affinity of these proteins for DNA is thought to be due to a "tail" at the N terminus of the peptide that is rich in positively charged residues providing a favorable electrostatic interaction (179)(180)(181). Dps proteins protect against multiple stress factors but require both DNA-binding and ferroxidase activity in all cases (181). We note that homologues of Dps proteins have been identified in nutritionally deficient stationary phase cultures that exhibit antioxidant activity but do not bind to DNA. These proteins, termed Dpr, are under the control of transcriptional regulators that respond to redox status/oxidative stress (e.g. PerR in Streptococcus pyogenes (182) or RitR in Streptococcus pneumoniae (183)).
The subunit arrangement of Dps 12-mer mini-ferritins results in a change in the symmetry of the channels penetrating the protein coat (2-fold channels and two classes of 3-fold channel) compared with the 24-mer proteins. One of the classes of 3-fold channel is unique to these proteins, whereas the second is similar to the 3-fold channels of other ferritins and is thought to constitute the route of iron entry (179,184). The location and structure of the ferroxidase center is also unique among the cage-forming ferritins. Rather than being buried within the four-a-helical bundle, it is located at the interface between the two protomers of each subunit dimer. In the majority of structural models derived from diffraction data, this site contains only a single ion coordinated by conserved carboxylate and histidine residues (184)(185)(186). The first reported example was from the Dps of Listeria innocua, with iron ions coordinated by Glu-62 and Asp-58 of one protomer and His-31 of its partner within the subunit dimer (184) (Fig. 5B).
A di-iron form of the catalytic site, modeled by placing an iron ion at the position of a nearby ordered water, suggested that Glu-62 might bridge the two metals, with His-43 from the same protomer as His-31 being the only other potential ligand. In the few cases where two metal ions have been observed at the ferroxidase center, the second metal has a significantly larger temperature factor than its surroundings, indicating significant lability of this site (187). Attempts to assess iron binding by fluorescence quenching indicated 24 eq of iron per protein upon the addition of Fe 31 but only 12 eq when titrating with Fe 21 (188). This has led to the proposal that the di-iron site is only formed as an intermediate in the oxidation reaction of Dps, in contrast to the 24-mer cages, where the occupancy of both sites is thought to be a prerequisite for rapid reactivity with either O 2 or H 2 O 2 . Consistent with a role in combating oxidative stress, the Dps centers utilize H 2 O 2 as the co-substrate for Fe 21 oxidation, being significantly less reactive toward O 2 (189).

Iron storage in cyanobacteria
A survey of the distribution of iron storage proteins in cyanobacterial genomes revealed significant differences from other bacteria, with only around 12% of genomes containing a homolog of FtnA. A great many of the genomes of marine picocyanobacteria (Prochlorococcus and Synechococcus) contain a distinct class of ferritin that differs from the classic Ftn proteins in that the coordinating side chains that make up site C are absent in the peptide chain. An example from Synechococcus sp. CC9311, SynFtn, was found to be up-regulated in response to exposure to elevated concentrations of copper (88). Furthermore, several of the marine picocyanobacteria possess genes encoding homologs of both SynFtn and FtnA. Together, these observations suggest that SynFtn may have a role in oxidative or general stress response rather than iron homeostasis. In vitro characterization of this protein demonstrated that, whereas the mineral core is generated via the typical displacement of oxidized iron from the catalytic center, the oxidation of this site proceeds via a mixed valent Fe 21 (190).
Whereas the genomes of many cyanobacteria lack homologs of any of the characterized 24-mer ferritins, homologs of the mini-ferritins appear to be widespread (191), and these have been shown to have roles in iron homeostasis, in addition to oxidative stress response (192). Some genomes encode multiple examples. Among the most extensively studied are those of Nostoc punctiforme, a filamentous cyanobacterium in which the majority of cells in filaments are in a vegetative state and perform photosynthesis, but around 5% form heterocysts-differentiated cells that perform an N 2 -fixing function. N. punctiforme encodes five Dps homologs (193), annotated NpDps1-5 (194), that are differentially transcribed, depending on cell type. Of these, NpDps1-3 have been designated typical Dps-like proteins based on sequence homology (195), with NpDps2 predominantly expressed in photosynthetic vegetative cells and the others predominantly in heterocysts. As with the Dps proteins of pathogens, they also use H 2 O 2 as the preferred oxidant. Whereas this group of proteins exhibit some degree of co-regulation, individual proteins are also thought to be up-regulated in response to a variety of environmental cues.
NpDps1 is expressed in response to low temperature (196), whereas NpDps2 confers resistance to oxidative stress induced both by exogenous H 2 O 2 (191) and high light levels (194) and is also expressed in response to heat shock. NpDps5 appears to perform a similar role to NpDps2, conferring resistance to both H 2 O 2 (197) and light-induced oxidative stress (194), but is also involved in iron homeostasis. The ligation of the ferroxidase center in this protein differs markedly from canonical Dps proteins and closely resembles that of bacterial Bfrs discussed above (191). Finally, NpDps4 possesses unusually His-rich ligation of iron at the ferroxidase center and utilizes only O 2 and not H 2 O 2 as an oxidant for iron (198). Accordingly, a role for this protein has been proposed as an O 2 scavenger within heterocysts where nitrogenase activity requires that a microoxic (,10 mM O 2 ) environment be maintained (199). Based on sequence comparisons with other Dps proteins, it has been suggested that this type of reaction center, which is common among, but restricted to, the cyanobacteria (198) be classified as the His-type ferroxidase center.

Iron storage in encapsulins
Encapsulins are large macromolecular assemblies, similar in structure to virus capsids. They are composed of proteins possessing the HK97 fold, a ubiquitous fold among proteins forming virus shells and other large compartments. (200). Two major classes of encapsulin cage architecture have been reported, distinguished by their triangulation number, T. The faces of the encapsulin are composed of regular hexagonal and pentagonal units, with curvature to create the enclosed 3D structure introduced by the latter. T defines the distance separating pentagonal units and therefore the size of the protein cage. Those with triangulation number T = 1 have the smallest possible enclosed volume and are composed of 60 identical subunits (201), whereas larger cages composed of 180 subunits possess a triangulation number T = 3 (202). Diameters range from 24 to 32 nm. Very recently, a new type of encapsulin was reported, from the bacterium Quasibacillus thermotolerans, which is larger still, with a diameter of 42 nm and novel T = 4 topology (203).
These large assemblies have the ability to encapsulate cargo proteins, which are targeted to the capsid by short C-terminal sequences (204). Among the cargo proteins of encapsulins are ferritin-like proteins. These encapsulated ferritins (EncFtn) are members of the ferritin superfamily that possess ferroxidase activity but do not themselves assemble to form cages (205). Whereas they are not as ubiquitous as their cage-forming counterparts, they have been identified in a wide range of bacterial and archaeal species from diverse environments (206). In all cases, these EncFtn proteins assemble into dimers; most assemble further to form annular pentamers of dimers (Fig. 6B). As a result, all lack the intrinsic ability to solubilize mineral cores, requiring localization within encapsulin cages to do so (207,208). Due to their greater size, encapsulin complexes containing EncFtn are capable of storing at least 4 times (205,207) (and in the case of the Q. thermotolerans encapsulin, ;10 times) the amount of iron associated with the classical ferritins described above.
Most EncFtn proteins differ from the other members of the ferritin superfamily in that the protein monomer essentially consists of two antiparallel a-helices, with an additional shorter helix at the C terminus. The classic four-a-helical motif of the ferritins is achieved by the association of these subunits into dimers. The Q. thermotolerans EncFtn is distinct in that its subunit consists of a four-a-helical bundle, which assembles into dimers.  (136), here colored green and cyan. In the true, cage-forming ferritins, these are connected via a loop joining helices B and C. Short helices running perpendicular to the long axis of the bundle help to template cage formation in the mini-ferritins (top) or 24-meric examples (middle). Members of the superfamily that do not form cages, such as EncFtn (bottom), are associated with further extended secondary structure elements, such as the membrane-spanning helices of MbfA or the large additional helices of EncFtn, which prevent assembly into cages. B, the annular pentamer of dimers adopted by the majority of encapsulated ferritins. C, the ferroxidase center of a typical encapsulated ferritin highlighting the noncrystallographic 2-fold symmetry of the iron environment. For clarity, only the ligands provided by the lower of the two protomers have been labeled. Images produced using PDB deposition 5N5E (206) (EncFtn).
The di-iron ferroxidase center has an approximate 2-fold symmetry axis (Fig. 6C), with each of the two monomers contributing identical ligand sets (cf. the case with the Dps proteins). In Q. thermotolerans EncFtn, each iron is coordinated by a bridging Glu and two His residues. In most others, each monomer provides a bridging Glu such that there are two equivalent Glu residues bridging the metals. Each iron is also ligated by a His and a bidentate Glu, with the two additional ligands located on the same monomer. The hydroxyl of a Tyr residue is located 4.5 Å from each of the irons of the ferroxidase center in most structures, but their significance is not known, as the mechanism of iron oxidation at EncFtn centers remains to be elucidated (206). Whereas these proteins have been demonstrated to support the catalytic oxidation of Fe 21 in the presence of O 2 , and this has been shown to be inhibited by Zn 21 , it is not known whether O 2 or H 2 O 2 is the preferred substrate of EncFtn.

Efflux of iron from the cell
Because iron has long been viewed as a growth-limiting nutrient, mechanisms of iron export from bacterial cells are a relatively underresearched area. However, it is apparent that under certain circumstances, simply down-regulating iron acquisition may not be sufficient to ensure cellular survival. Chief among these is ROS assault, which arises from the close link between oxidative stress and elevated levels of intracellular iron mediated by the Fenton reaction. In some cases at least, countering this assault necessitates the active removal of iron from the cell, but the discovery of the efflux systems responsible is a relatively recent development (209). Consequently, the understanding of these systems lacks the mechanistic detail available for the molecules of iron sensing, import, and storage. However, the main features of the four known classes of bacterial iron efflux systems are outlined below. P-type ATPases are cytoplasmic membrane proteins that consist of a transmembrane domain containing 6-8 helices, an ATP-binding domain, and a soluble actuator domain. Examples with iron-exporting activity belong to the P 1B4 family and have been identified in Bacillus subtilis (PfeT) (210), Listeria monocytogenes (FrvA) (211), M. tuberculosis (CtpD) (212), the group A Streptococci (PmtA) (213,214), and Sinorhizobium meliloti (Nia) (215). Where the regulator of transcription has been identified, it is Fur and/or PerR, indicating the dual role in ironmediated and peroxide stress response.
Cation diffusion facilitator (CDF) metal ion transporters are ubiquitous among prokaryotes and eukaryotes, with a wide range of cations transported. The proteins consist of six transmembrane helices with a histidine-rich loop interconnecting transmembrane helices 4 and 5. A soluble cytoplasmic domain is located at the C terminus. Little is known about the factors influencing metal ion selectivity, but ironexporting activity has been reported for examples from E. coli (YiiP or FieF) (216), P. aeruginosa (AitP) (217), and Shewonella oneidensis (FeoE) (218). Unlike the P-type ATPase systems, the transcriptional regulators of their expression have yet to be identified.
Major facilitator superfamily proteins function in the transmembrane transport of cations, but the mechanism by which they achieve this is not well-understood. They are made up of two domains, each consisting of six transmembrane helices. IceT of Salmonella typhimurium (219) is the only reported example with iron-exporting activity and is under the transcriptional control of the BaeSR system that regulates antibiotic resistance and efflux.
Membrane-bound ferritins do not form cages and are therefore are not bona fide ferritins (Fig. 6A). However, they contain a ferritin-like domain at the N terminus that has ferroxidase activity (220). Located on the cytoplasmic side of the membrane, this domain is required for iron transport. The Cterminal domain is membrane-spanning and has significant sequence homology to the vacuolar iron transporters, such as

Concluding remarks
In this review, we have attempted to provide an overview of the current understanding of iron detoxification by bacteria, as summarized in Fig. 7. The modes of operation of the Fe 21binding transcriptional regulators Fur and DtxR are now understood in molecular detail, and a great many genes under their control have been identified. Work is now under way unraveling the complex interplay between these and other regulators involved in response to oxidative and nutritional stress, and a great deal of progress is being made in this area. Whereas no crystal structures are yet available for the ironresponsive transcriptional regulators of the a-proteobacteria, Irr and RirA, the mechanism by which they use iron-containing prosthetic groups to sense the concentration of the metal has been established, as has the molecular basis of their ability to also sense O 2 . Also, an understanding of the interplay between these two regulators and the genes that they control is emerging. The common thread between all is a down-regulation of iron acquisition pathways and up-regulation of iron storage systems in response to elevated iron concentrations (Fig. 7).
The greater number of ferritins encoded in bacterial genomes compared with those of animals possibly reflects the greater need for bacterial cells to respond to a variety of environmental stresses that are linked to iron, from iron deprivation to ROS-and RNS-induced oxidative stress. Reported growth inhibition of deletion mutants compared with WT strains of various bacteria consistently supports the notion that ferritin minerals are viable stores of nutritionally available iron.
A recent study of E. coli revealed that exponentially growing cells contain a significant proportion of iron in the reduced state, with ferric mineral iron only accumulating in stationary phase (131). This fascinating result highlights the importance of precise physiological conditions in determining the extent to which the quota of iron within E. coli cells is oxidized to the ferric state. It suggests that the redox state of intracellular iron in bacterial cells is a more subtle balance of the oxidoreductase activity of ferritins and the reducing environment created by low-molecular weight thiols than has previously been appreciated. These observations were rationalized in terms of an expansion of the "respiratory shield" hypothesis originally proposed for mitochondria. In essence, diffusion of O 2 across either the mitochondrial or, in this instance, the cytoplasmic membrane is prevented by its consumption during respiration. Thus, the enzymes of the respiratory chain form a shield, creating a microaerobic environment in the interior matrix/cytoplasm that protects O 2 -sensitive proteins and cofactors from damage during normal respiratory function. The static dissolved O 2 concentration inside mitochondria has been estimated at around 1 mM (131), and that in the cytoplasm of bacterial cells is assumed to be similar during exponential growth. This emphasizes an important difference between the environments in which the ferritins of bacteria and animals operate. Respiration in animal cells is restricted to mitochondria, and ferritins located in the cytosol are therefore exposed to a significantly greater O 2 concentration than their bacterial counterparts for which peroxide would logically be expected to be an available co-substrate for iron oxidation.
A topical debate in the field of ferritin research is the existence or otherwise of a 'universal' mechanism of iron oxidation. This was proposed based on similarities between different ferritins in terms of their mineralized iron products, their iron-binding stoichiometries, and common intermediates that are formed during Fe 21 oxidation/mineralization (222). The above considerations would argue for variation between bacterial and animal ferritins based on availability of potential substrates. Furthermore, the existence of multiple welldescribed mechanisms, including the very recent discovery of extremely unusual iron-O 2 chemistry in the cyanobacterial ferritin SynFtn (190), which share only the broadest characteristics, provides ample evidence that such variation exists even within bacterial ferritins. Nature never fails to impress with the different ways in which it has found solutions to similar, if not identical, problems. The encapsulated ferritins provide the most recently discovered and a particularly striking example of the variety of solutions to the problems posed by iron. Figure 7. Schematic overview of the major components of iron sensing and detoxification found in bacterial cells. Note that not all of these components are present in a single bacterial cell. Regulatory proteins are shown here as repressors but, in some cases, can also act as activators. Encapsulins are large protein compartments that house EncFtn ferritin-like proteins. The fate of iron stored in encapsulins and in Dps proteins is not clear, although it is likely that at some point, it becomes bioavailable again. Ftn, Bfr, and Dps do not appear to be distributed according to phyla. Fur is the transcriptional regulator in most bacteria but is replaced by DtxR/IdeR in some actinobacteria. In the a-proteobacteria, Fur plays a diminished role in iron homeostasis, with the majority of these functions being performed by Irr. In some rhizobiales, this is achieved in conjunction with a second global regulator, RirA. Import of siderophores and heme across the cytoplasmic membrane (IM) is performed by ABC transporters in all known cases, and Feo is the major importer of Fe 21 . In Gram-negative bacteria, heme and siderophores are imported to the periplasm by outer-membrane (OM) porins, whereas a network of heme-binding proteins transports this cofactor across the cell wall of the Gram-positive bacteria. Characterized Fe 21 export systems are rare, but P-type ATPases are the most widely distributed. IceT of S. typhimurium is the only example of the MFS characterized to date, whereas the CDF proteins are limited to g-proteobacteria and the MbfA proteins to a-proteobacteria. YiiP from E. coli is the only Fe 21 efflux pump for which the structure has been solved (227).
University of Essex for supporting our work on ferritins and other iron metabolism proteins over many years.
Funding and additional information-This work was supported by Biotechnology and Biological Sciences Research Council Grants BB/R002363/1 and BB/R003203/1.
Conflict of interest-The authors declare that they have no conflicts of interest with the contents of this article.