The IL1α-S100A13 Heterotetrameric Complex Structure

Interleukin 1α (IL1α) plays an important role in several key biological functions, such as angiogenesis, cell proliferation, and tumor growth in several types of cancer. IL1α is a potent cytokine that induces a wide spectrum of immunological and inflammatory activities. The biological effects of IL1α are mediated through the activation of transmembrane receptors (IL1Rs) and therefore require the release of the protein into the extracellular space. IL1α is exported through a non-classical release pathway involving the formation of a specific multiprotein complex, which includes IL1α and S100A13. Because IL1α plays an important role in cell proliferation and angiogenesis, inhibiting the formation of the IL1α-S100A13 complex would be an effective strategy to inhibit a wide range of cancers. To understand the molecular events in the IL1α release pathway, we studied the structure of the IL1α-S100A13 tetrameric complex, which is the key complex formed during the non-classical pathway of IL1α release.

Interleukin 1␣ (IL1␣) and interleukin 1␤ (IL1␤) are prototypic members of the IL1 gene family; these proteins are well recognized for their inflammatory and angiogenic activities (1)(2)(3)(4). IL1␣ is a potent cytokine that possesses a wide spectrum of immunological and inflammatory activities (5,6). It is also involved in pathological processes such as restenosis and tumor formation (7,8). IL1␣ is synthesized as a higher molecular weight precursor protein, pIL1␣, which is cleaved by calpain or a calpain-like protease to form mature IL1␣. The biological functions of IL1␣ are mediated by binding to transmembrane receptors and thus require the export of mature IL1␣ to the extracellular space (9,10).
In general, proteins are exported via the ER-Golgi pathway with the help of a hydrophobic N-terminal sequence, which allows the protein to enter the ER-Golgi pathway (11). However, several extracellular proteins such as IL1␣, IL1␤, fibroblast growth factor (FGF1 and FGF2), S100A13, sphingosine kinase 1, gelectin-1, and the extra vesicular p40 fragment of Syt1 all lack N-terminal signal sequences and are exported via ER-Golgi independent non-classical routes (10,(12)(13)(14). Most of these proteins are involved in several key biological func-tions, such as angiogenesis, cell proliferation or differentiation, and tumor growth. It was first assumed that angiogenic growth factors could be released from mechanically injured tissues to promote wound healing, a process that requires angiogenesis. Various lines of evidence demonstrated that these proteins are exported from cultured cells in the absence of cell death (15,16). IL1␣ is secreted by a different pathway, which is activated by diverse forms of stress, including heat shock (15,16) and serum starvation (17). Thus, it is important to understand and define the non-classical pathway responsible for the export of IL1␣; this information may eventually help in the clinical management of inflammatory and angiogenic-dependent events. In this article we discuss the molecular interactions in the nonclassical secretory pathway of interleukin 1␣.
IL1␣ and FGF1 are structurally homologous proteins, which are exported via an ER-Golgi independent pathway (18). In both cases, a multiprotein complex forms and traverses the membrane. Unlike FGF1, IL1␣ release does not depend on the covalent dimerization of IL1␣. Mature IL1␣ is exported as a monomeric, biologically active cytokine (19,20). In this nonclassical secretion pathway, FGF1 associates with S100A13 and Syt1, whereas IL1␣ associates with S100A13; these protein complexes then bind to copper ions before being secreted. IL1␣ and the Ca 2ϩ binding protein S100A13 associate in the cytoplasm (19,20). Apparently, both proteins are secreted together. Direct roles for S100A13 in the export of IL1␣ have been demonstrated by the expression of a dominant-negative S100A13 mutant that attenuates the export of IL1␣ (19). S100A13 is a member of the S100 protein family. It is characterized by its specificity for diverse forms of cancer (21)(22)(23)(24). S100A13 acts as a template molecule for the non-classical secretion pathway of IL1␣, FGF1 (25), and prothymosin-␣ (26). S100A13 has been reported to co-express with IL1␣ in brain tumors, demonstrating a perivascular distribution. S100A13 is a member of the family of Ca 2ϩ -binding proteins that is characterized by the absence of a classical signal peptide sequence and the presence of two EF-hand domains. S100A13 is a novel member of the S100 gene family, which encodes a highly charged carboxyl-terminal domain that could be involved in specific protein interactions. S100 proteins are characterized by two distinct EF-hand motifs that have different Ca 2ϩ affinities (27). Maciag and co-workers (19,20) demonstrated that S100A13 is involved in regulating the release of IL1␣ in response to stress, independent of the conventional ER-Golgi pathway. When S100A13 is expressed in IL1␣-free cells, it is spontaneously released via a non-classical pathway at both at 37 and 42°C; however, when it is expressed in cells with IL1␣, it is released only in response to heat shock (19). An additional indi-cation of the participation of S100A13 in the export of proinflammatory cytokines is its specificity for binding to antiinflammatory drugs such as amlexanox and chromolyn (28). S100A13 appears to be the central player in the formation of this complex.
These results suggest that IL1␣-S100A13 complex formation is the first step in the export of IL1␣, followed by direct translocation of this protein complex across the plasma membrane. In this article, we describe the interfacial regions of the solution structures of free IL1␣ and the IL1␣-S100A13 complex. Our results demonstrate that S100A13 acts as a template for the formation of the multiprotein complex.

EXPERIMENTAL PROCEDURES
Ingredients for Luria Broth were obtained from AMRESCO. Aprotinin, pepstatin, leupeptin, phenylmethylsulfonyl fluoride, Triton X-100, and ␤-mercaptoethanol were obtained from Sigma. Heparin and glutathione-Sepharose were obtained from Amersham Biosciences. 15 NH 4 Cl, 13 C-labeled glucose, and D 2 O were purchased from Cambridge Isotope Laboratories. All other chemicals used were of high quality analytical grade. Unless specified, all solutions were made in 25 mM sodium phosphate containing 100 mM NaCl and 2 mM CaCl 2 .
Expression and Purification of the IL1␣ and S100A13-Human IL1␣ cDNA encoding the recombinant protein was subcloned into a pET(20bϩ) expression vector. IL1␣ was overexpressed and purified as described by Chang et al. (29). Human S100A13 cDNA encoding the recombinant protein was subcloned into a pGEX-4T1 expression vector. S100A13 was expressed in Escherichia coli BL21(DE3). The unlabeled protein was expressed in Luria broth (LB) medium. The soluble portion of the cell lysate was loaded onto a GST-Sepharose column. Nonspecifically bound proteins were removed by washing the column with PBS. The bound GST-S100A13 protein was eluted with 10 mM glutathione and 50 mM Tris-HCl (pH 8.0). The GST-fused S100A13 protein was exchanged into PBS, and the solution was treated with 50 g of thrombin for 10 -12 h to cleave the GST fusion protein. The protein was reloaded onto a GST column to obtain pure S100A13. The S100A13 and IL1␣ were further purified by gel filtration on a Superdex-75 (GE Healthcare) column with 25 mM sodium phosphate (pH 6.5) containing 100 mM NaCl and 2 mM calcium chloride as the eluent. The purity of the protein was verified by SDS-PAGE, and the molecular weight was confirmed by electrospray mass spectrometry.
Preparation of Isotopically Enriched IL1␣ and S100A13-Uniform 15 N and 15 N, 13 C labeling of IL1␣ and S100A13 was achieved by culturing the cells in M9 minimal medium containing either 15 NH 4 Cl for single ( 15 N) labeling or 15 NH 4 Cl and 13 C glucose for double ( 15 N and 13 C) labeling. To achieve maximal expression yields, the composition of the M9 medium was modified by the addition of a vitamin mixture. The expression host strain E. coli BL21(DE3) pLysS is a vitamin B1-deficient host; therefore, the medium was supplemented with thiamine (vitamin B1).
Isothermal Titration Calorimetry-Protein-protein binding was characterized by measuring the change in heat caused by titration of one binding partner into a solution containing the other binding partner, using a Microcal VP titration calorimeter at 25°C. IL1␣ and S100A13 solutions (25 mM sodium phosphate (pH 6.5), 100 mM NaCl, and 2 mM calcium chloride) were centrifuged and degassed under vacuum before use. Titrations were performed by injecting 8-l aliquots of protein (30 times; 0.1 mM) into 0.01 mM of the binding partner. The titration curves were corrected using buffer-protein and protein-buffer controls and analyzed using Origin software supplied by Microcal.
1 H-15 N HSQC 2 Titration-NMR data were recorded at 25°C on a Varian 700 MHz spectrometer equipped with a cold probe. For the two-dimensional heteronuclear experiments, the concentration of the proteins was ϳ0.5 mM. All protein samples were prepared in 25 mM phosphate buffer (pH ϳ 6.5, 90% H 2 O, 10% D 2 O) containing 100 mM NaCl and 2 mM CaCl 2 . The 15 Nlabeled proteins were titrated with unlabeled protein at a 1:1 molar ratio. The weighted average of the 15 N and 1 H chemical shift perturbations was calculated using the equation ⌬␦ ϭ [(␦ 1 H) 2 ϩ 0.2 (␦ 15 N) 2 ] 1/2 . Amide proton exchange rates were monitored by acquiring a series of 1 H-15 N HSQC spectra of the free protein and the protein in complex with other proteins involved in the multiprotein release complex. The spectra were processed with V-nmr and analyzed with SPARKY (30).
Structure Calculations-The free IL1␣ structure and structures of IL1␣ and S100A13 in the IL1␣-S100A13 tetrameric complex were calculated iteratively with ARIA/CNS (version 2.2) using the PARALLHDG 5.3 force field in the PARALLHDG mode (37,38). Preliminary structure calculations based on intramolecular NOE data and TALOS (39) data established that the backbone folds of IL1␣ and S100A13 are not substantially altered in the protein complex. Distance restraints, dihedral angles, and hydrogen bond constraints were used in the structure calculation. Interproton distance restraints for the structure calculations were derived from the 15 N-separated NOESY-HSQC and 13 C-separated NOESY-HSQC experi-ments. The quality of the calculated structures was assessed using PROCHECK (40).
Docking Studies-HADDOCK (41)(42)(43)(44)(45) was used to dock IL1␣ and S100A13 in the tetrameric complex using the previously determined structures established by ARIA and intermolecular NOEs. Intermolecular distance restraints were derived from a three-dimensional 13 C and 15 N (F 1 )-filtered, 13 C (F 2 )edited, and 12 C (F 3 )-filtered NOESY experiment. A scaling factor was determined by comparing the intensities of the resolved peaks with those of the corresponding peaks in the 13 C-edited NOESY spectrum acquired for the IL1␣-S100A13 complex. The chemical shift perturbations observed upon complex formation were used to define ambiguous interaction restraints for residues at the interface. Active residues were defined as those having both chemical shift perturbations and a relative residue accessible surface area larger than 50% for either side chain or backbone atoms as calculated by NACCESS (46). Passive residues were defined as all other surface non-accessible residues (having a relative residue accessible surface area smaller than 50% for side chain or backbone atoms). Ambiguous interaction restraints were defined between every active residue of the first protein and all active and passive residues of the second protein and vice versa. A total of 5000 rigid-body docking trials were carried out using the standard HADDOCK protocol. The 100 lowest energy solutions were used for subsequent semiflexible simulated annealing and water refinement. The 20 lowest energy structures were used to represent the structure of the complex. The structures were analyzed with PROCHECK (40).

RESULTS
In this report, we studied the solution structure of free IL1␣ and its interactions with S100A13 by ITC and NMR. We solved the solution structure of the IL1␣-S100A13 complex, a key component in the non-classical secretion pathway of IL1␣. We also studied the interaction of amlexanox with IL1␣, which is known to inhibit the non-classical secretion pathway of IL1␣ and S100A13.
Structure of Free IL1␣-The complete resonance assignments of free IL1␣ were deposited in the BMRB under the accession number 16379. A set of 1536 intramolecular NOEs was assigned from the three-dimensional 15 N-edited NOESY-HSQC and converted into 1458 relevant distance restraints. In addition, 62 hydrogen bonds, identified from deuterium exchange experiments, were also used. Thus, a total of 1520 distance restraints were used in the final structure calculations (Table 1). Fig. 1A shows the superposition of the 20 lowestenergy structures. (The coordinates of these structures have been deposited in the Protein Data Bank as 2KKI.) The average r.m.s. deviation value for the secondary structure region was 0.38 Ϯ 0.02 Å for the backbone atoms and 0.81 Ϯ 0.02 Å for all heavy atoms. Analysis of the structures by PROCHECK indicated good stereochemistry for the bond angles and bond lengths and showed that 98.6% of all of the non-glycine residues fall within the allowed region of the Ramachandran plot (Table 1). Fig. 1B shows the superposition of an ensemble of 20 structures of free IL1␣. The free IL1␣ structure contains 13 ␤-strands (␤ 1 , 12-26; ␤ 2 , 31-34; ␤ 3 , 40 -43; ␤ 4 , 54 -60; ␤ 5 , 69 -74; ␤ 6 , 78 -82; ␤ 7 , 90 -93; ␤ 8 , 101-102; ␤ 9 , 111-116; ␤ 10 , 119 -124; ␤ 11 , 131-132; ␤ 12 , 142-143; ␤ 13 , 151-155) and one ␣-helix (␣ 1 , 104 -109). The chemical shift index of free IL1␣ was calculated using the H ␣ , C ␣ , C ␤ , and CЈ resonances (47). The consensus chemical shift index (supplemental Fig. S1) also indicated that IL1␣ consists of 13 ␤-strands connected by turns. Although the position of the ␤-strands were shifted, truncated, or elongated by one or two residues, the predicted secondary structure of IL1␣ from the chemical shift index agreed well with the secondary structure observed in the structure. According to the crystal structure, IL1␣ contains 12 ␤-strands. The range of ␤-strands in the NMR structure is almost the same as in the x-ray structure. The core of this structure is a 6-stranded ␤-barrel that is closed at one end by another 6 ␤-strands to form a bowl-like structure. The main feature of the structural core is that it has nearly precise internal 3-fold symmetry. The r.m.s. deviation difference between the solution structure and the x-ray structure (48) for the backbone atoms was 1.31 Å.
IL1␣-S100A13 Binding Studies-Isothermal titration calorimetry (ITC) is a useful technique for the study of protein-  protein interactions (49). ITC can be used to reliably measure the binding constants and energy changes that accompany protein-protein binding. Most importantly, ITC provides information on the number of protein binding sites. We measured the binding affinities of IL1␣ to S100A13 and S100A13 to IL1␣ by ITC (Fig. 2). The binding constants are in the range of 2.15-3.23 ϫ 10 Ϫ6 M. Chemical shift perturbation is the most commonly used NMR method to map protein interfaces (50,51). The crosspeaks in the 1 H-15 N HSQC spectrum that are perturbed upon addition of the unlabeled protein often represent the binding site(s) of the protein to the target protein. Therefore, monitoring 1 H-15 N chemical shift perturbations by observing the shifts in the 1 H-15 N HSQC spectrum provides residue-level information about the protein-protein interface. The interaction causes changes in the chemical environment of the protein interface and therefore affects the chemical shifts of the nuclei in that area. 15 N chemical shift perturbation has been used in many cases to map protein-protein interactions (50 -52). To understand the mechanism of the IL1␣ non-classical secretion pathway at the molecular level, we solved the structures of free IL1␣ and the IL1␣-S100A13 tetrameric complex, which is potentially the key complex formed in the non-classical secretion pathway of IL1␣.
Comparison of the H/D exchange rates of the individual amide protons in the free and bound states confirmed the binding interfaces in the IL1␣-S100A13 complex. Amide protons in proteins can readily exchange with deuterons. However, amide protons that are involved in backbone hydrogen bonds are more resistant to exchange than those in the unstructured portions of the protein (53). After mapping the interfaces using chemical shift perturbation and H-D exchange data, we focused on solving the solution structure of the IL1␣-S100A13 tetrameric complex to understand the molecular interactions in more detail.
Structure of the IL1␣-S100A13 Tetrameric Complex-The resonance assignments of the IL1␣-S100A13 tetrameric complex were obtained using standard strategies based on tripleresonance experiments. In total, 95% of the backbone amide resonances in the 1 H-15 N HSQC spectrum and their ␣ carbons were sequentially assigned based on HNCA and HN(CO)CA experiments. The backbone carbonyl carbons were assigned using the HNCO experiment. The H ␣ and side chain proton resonances were assigned based on three-dimensional 15 N-edited TOCSY-HSQC, three-dimensional HCCH-TOCSY, and 15 N-edited NOESY-HSQC experiments. The NH 2 groups of Gln and Asn residues were connected to their side chain ␥ and ␤ protons using an 15 N-edited NOESY-HSQC. The Structure of IL1␣ in the IL1␣-S100A13 Complex-A set of 1596 intramolecular NOEs of IL1␣ were assigned from the three-dimensional 15 N-edited NOESY-HSQC and converted to 1510 relevant distance restraints. In addition, 60 hydrogen bonds, identified from deuterium exchange experiments, were also used. Thus, a total of 1570 distance restraints were used in the final structure calculations ( Table 2). The final representative ensemble of structures shows few molecular and constraint violations greater than 5 Å. The average r.m.s. deviation value for the secondary structure region was 0.35 Ϯ 0.02 Å for the backbone atoms and 0.84 Ϯ 0.03 Å for all heavy atoms. Analysis of the structures by PROCHECK indicated good stereochemis-try for the bond angles and bond lengths and that 98.8% of all of the non-glycine residues fall within the allowed region of the Ramachandran plot (Table 2). Fig. 3A shows the superposition of an ensemble of 20 structures of the IL1␣ in IL1␣-S100A13 complex. The IL1␣ structure contains 13 ␤-strands and one ␣-helix. The structure of IL1␣ in the complex is similar to that in the unbound state. The S100A13 binding site on IL1␣ is distributed over four regions. The first region is in the ␤ 3 strand, the second region is loop 6 and ␤ 7 , the third region is in helix 1, and the fourth region is in loop 9. The majority of the residues in the interfacial region are highly solvent accessible. When the structures of the free (Fig.  1A) and bound (Fig. 3A) IL1␣ are compared, some differences are seen in the binding interface, possibly attributable to the binding of S100A13. The ␤ 3 strand, loop 6, and ␤ 7 strand of IL1␣ are near the C terminus of S100A13. Loop 9 is near loop 3, helix 1 is near loop 1, and loop 6 is near loop 1 and helix 1 of S100A13.
The Structure of S100A13 in the IL1␣-S100A13 Complex-A set of 1656 intramolecular NOEs of S100A13 were assigned from the three-dimensional 15 N-edited NOESY-HSQC spectrum and converted to 1625 relevant distance restraints. Additionally, 48 hydrogen bonds identified from the deuterium exchange experiments were also used. Thus, a total of 1673 distance restraints were used in the final structure calculations ( Table 2). The 20 lowest energy structures of the S100A13 homodimer (Fig. 3C) complex with IL1␣ were used to represent the structure of the tetrameric complex. The average r.m.s. deviation value for the secondary structure region was 0.38 Ϯ 0.02 Å for the backbone atoms and 0.87 Ϯ 0.03 Å for all heavy atoms. Analysis of the structures by PROCHECK indicated good stereochemistry for the bond angles and bond lengths and showed that 99.2% of all of the non-glycine residues fall within  B, ribbon representation of IL1␣ in the IL1␣-S100A13 tetrameric complex. C, superposition of the backbone (N, C ␣ , and CЈ) atoms of the 20 final solution structures of the S100A13 dimer in the IL1␣-S100A13 tetrameric complex. The two monomers are colored green and brown. D, ribbon representation of S100A13 in the IL1␣-S100A13 tetrameric complex.

IL1␣-S100A13 Heterotetrameric Complex Structure
the allowed region of the Ramachandran plot. Fig. 3C shows the superposition of an ensemble of 20 structures of S100A13 in the tetrameric complex with IL1␣. The S100A13 structure is a homodimer, with each monomer composed of four ␣-helices and two ␤-strands, in agreement with the chemical shift indices. The IL1␣ binding site on S100A13 is distributed over four regions. The first region is in helix 1, the second region is in loop 1, the third region is in loop 3, and the final region is in the basic C terminus. The majority of the residues in the interfacial region are highly solvent accessible. Structure of IL1␣-S100A13 Complex-Triple resonance experiments were performed by mixing double-labeled ( 15 N and 13 C) protein with the corresponding unlabeled partner(s) to form the appropriate complexes. We assigned the IL1␣ and S100A13 resonances in the IL1␣-S100A13 tetrameric complex. An unambiguous method for mapping biomolecular interactions is to use the intermolecular nuclear Overhauser effect (NOE) (54). The intensity of the NOE is proportional to the sixth root of the inter-proton distance (r Ϫ6 ). We obtained intramolecular NOEs from isotope-edited NOE spectra using 15 N-edited NOESY and 13 C-filtered NOESY experiments. We observed 46 intermolecular NOEs in the IL1␣-S100A13 (supplemental Fig. S4) complexes by mixing a double-labeled ( 15 N, 13 C) protein with its corresponding unlabeled protein partner(s).
Calculating the structures of protein-protein complexes using intermolecular data, chemical shift perturbation data, or both has recently been highly successful when using the modeling program HADDOCK (36 -40). HADDOCK was used to dock the previously determined structures of IL1␣ and S100A13 (in IL1␣-S100A13 complex) from ARIA/CNS. The structure of the binary complex of IL1␣ and S100A13 was calculated using 46 intermolecular NOEs obtained from the filtered NOE data of the complex. Based on the chemical shift perturbations of IL1␣ and S100A13 upon complex formation, ambiguous interaction restraints were defined for the residues at the interface. The active and passive residues were used to generate ambiguous interaction restraints (Table 3). A total of 5000 rigid body docking trials were carried out using the standard HADDOCK protocol with the 100 lowest energy structures used for subsequent semiflexible simulated annealing and water refinement. The 20 lowest energy structures were used to represent the structure of the complex (Fig. 4A). The average r.m.s. deviation value for the backbone is 0.41 Ϯ 0.03. The average r.m.s. deviation at the interface between IL1␣ and S100A13 is 0.52 Ϯ 0.05. The average r.m.s. deviation at the IL1␣ interface is 0.57 Ϯ 0.05 Å. The average r.m.s. deviation at the S100A13 interface is 0.59 Ϯ 0.05 Å. The complex structure was analyzed using PROCHECK (40). Fig. 4B shows the ribbon representation of the IL1␣-S100A13 tetrameric complex. S100A13 is a homodimer. In this complex, each monomer binds one IL1␣ molecule, and the complex appears as two symmetric units.

DISCUSSION
ITC provides direct information on the stoichiometry, binding affinity, and heat changes that occur during proteinprotein binding in solution. Here, we monitored the binding affinity of IL1␣ to S100A13 and S100A13 to IL1␣. The isotherms in Fig. 2 represent the binding between IL1␣ and S100A13. The binding constant between IL1␣ and S100A13 is rather weak (2.15-3.23 M, Fig. 2). The titration curve representing the binding of IL1␣-S100A13 saturates at a protein to ligand ratio of 1:1.  The green and maroon colors represent the S100A13 dimer and two IL1␣ monomers, respectively. B, ribbon and surface representation of the IL1␣-S100A13 tetrameric complex; IL1␣ is shown in maroon; the two S100A13 monomers are shown in green. C, the electrostatic representation of the IL1␣-S100A13 tetrameric complex shows that the binding between the two molecules is due to hydrophobic and charge-charge interactions.
The IL1␣-S100A13 Interface-A detailed summary of the intermolecular contacts between S100A13 and IL1␣ is shown in Fig. 5. There are 44 residues at the interface region; 25 are from S100A13, and 19 are from IL1␣. The majority of the interactions between the two proteins are either hydrophobic or electrostatic. Key residues that are involved in two or more intermolecular contacts include Phe 21 Ј, Thr 22 Ј, Ala 24 Ј, Arg 29 Ј, Lys 30 Ј, Ser 32 Ј, Arg 88 , and Lys 89 Ј of S100A13 and Thr 41 , Gln 84 , Asp 85 , Val 87 , Val 90 , Met 95 , Ser 105 , and Thr 107 of IL1␣. Based on these results, the contact between proteins S100A13 and IL1␣ is mainly the result of a combination of hydrophobic and polar interactions (Fig. 5B).
In addition, 24 hydrogen bonds between the side chains and the backbone were observed. The side chain of Arg 25 Ј(NE) forms a hydrogen bond with the backbone nitrogen of Val 90 . There are also hydrogen bonds between Arg 25 Ј(O) and Gln 88 HB1, Lys 30 Ј(O) and Leu 90 HD1, Lys 89 Ј OE1 and Ser 105 HB2, and Gly 104 O and Arg 88 Ј(HH2). The large interfacial region between IL1␣ and S100A13 (ϳ2652 Å) contains intermolecular salt bridges, hydrogen bonds, and hydrophobic contacts, all of which provide information for binding recognition between IL1␣ and S100A13.
Inhibition of IL1␣-S100A13 Complex Formation-Amlexanox is a drug known to inhibit the non-classical secretion FIGURE 5. A, summary of the intermolecular contacts between IL1␣ and S100A13 in the IL1␣-S100A13 complex. B, stereo view of intermolecular contacts (as indicated by dotted lines) between IL1␣ and S100A13 in the IL1␣-S100A13 heterotetrameric complex.
pathway of IL1␣-S100A13 (20,55). In this report, we investigated the amlexanox binding site on IL1␣ by titrating amlexanox with IL1␣ and monitoring the change in chemical shifts in the 1 H-15 N HSQC spectrum (Fig. 6A). Notably, the amlexanox binding site on IL1␣ is located in two regions. The major binding region is in the interface of the IL1␣-S100A13 complex (Fig.   6, B and C). Based on the above results and previous results (S100A13-amlexanox interaction), amlexanox can inhibit formation of the IL1␣-S100A13 complex.
The Mechanism for the Non-classical Secretory Pathway of IL1␣-In this article, we describe the solution structure of the IL1␣-S100A13 heterotetrameric complex, which is the core FIGURE 6. A, overlay of 1 H-15 N HSQC spectra of IL1␣ (uniformly labeled with 15 N) in its free state (black) and in the amlexanox-bound state (red). B, ribbon representation of IL1␣; the green region indicates the amlexanox binding sites on IL1␣. C, amlexanox chemical structure. D, ribbon representation of the IL1␣-S100A13 tetrameric complex structure. Amlexanox binding sites on IL1␣ are in red; the amlexanox binding site is in the interface of the IL1␣-S100A13 tetrameric complex, implying that amlexanox can inhibit formation of the tetrameric complex. component of the interleukin-1␣ non-classical secretion pathway. S100A13 plays a crucial role in the entire non-classical secretion pathway. S100A13 is a homodimer and acts as a template for binding two IL1␣ molecules to form the tetrameric complex. Copper also binds to S100A13 and IL1␣, which is essential for the non-classical secretion pathway of both proteins (19,56). Maciag et al. (10,19) described how copper plays a crucial role in the non-classical secretion pathway of IL1␣.
The formation of the multiprotein complex takes place in the vicinity of the cell membrane (10). The cell membrane is asymmetric, with acidic phospholipids such as phosphatidylglycerol, phosphatidylserine, and phosphatidylinositol in the inner leaflet of the cell membrane (57). Extracellular stimuli induce the flipping of acidic phospholipids to the cell surface (58) where phosphatidylserine can be detected using fluorescently tagged recombinant annexin V (59). This externalization mechanism is reversible. IL1␣ and S100A13 specifically bind acidic phospholipids and have been demonstrated to destabilize liposomes, which are composed of acidic phospholipids (19,60).
Based on the present results and evidence from the literature, we propose a mechanism for the non-classical secretory pathway of IL1␣. First, apo-S100A13 binds to Ca 2ϩ ions to form halo-S100A13, which is the active conformation for tetrameric complex formation. The active S100A13 then binds to IL1␣, which is the core component of the multiprotein complex. Formation of the tetrameric complex is the key step in the nonclassical secretory pathway of IL1␣. Later, this complex interacts with Cu 2ϩ ions (carried by SK1) and moves close to the acidic environment of the inner leaflet of the cell membrane. A conformational change could occur in the tetrameric complex due the acidic conditions in the inner leaflet and the presence of Cu 2ϩ ions. These partially structured states of the complex that are generated at the membrane are highly competent to traverse the membrane bilayer because the partial unfolding results in the exposure of normally hidden hydrophobic residues (61). IL1␣ is secreted as a monomer in an active conformation (19,20). Under reducing conditions, such as those found outside the cell membrane, the complex dissociates.
In this article, we elucidate the interfacial regions of the proteins in the tetrameric complex, which is the core component of the interleukin-1␣ non-classical secretory pathway. These findings may prove useful in understanding the mechanism of the non-classical pathway of IL1␣ secretion at the molecular level. The biological effects of IL1␣ are mediated through the activation of transmembrane receptors and therefore require the release of these proteins into the extracellular space. The information provided within this article might provide clues on how to stop the formation of the multiprotein complex, which is essential for IL1␣ transport, thereby assisting in rational drug design for IL1␣-induced angiogenesis and cell proliferation.