|
Table II
Methyltransferase activities in cell extracts of E. coli transformed
with E. halochloris and A. halophila GSMT and SDMT
The activity on glycine, sarcosine, and dimethylglycine is expressed as
micromoles of methyl groups transferred per min. The genes were
overexpressed under E. coli phage T5 promoter using pQE-60
expression vector. Initial reaction rates were measured. The errors
were within 15%.
|
|
| Gene |
Activity
units/ml
|
| Glycine |
Sarcosine |
Dimethylglycine |
|
| E.
halochloris |
| GSMT |
0.58 |
0.19 |
0
|
| SDMT |
0.006 |
1.3 |
5.6 |
| A. halophila
|
| SDMT-GSMT1-565a |
0 |
0.028 |
0.072
|
| GSMT1-281a
(truncated) |
0 |
0 |
0
|
| SDMT282-565a
(truncated) |
0.017 |
0.77 |
1.5 |
|
|
a
For the amino acid number of
truncated A. halophila proteins, see Fig. 2.
|
|