FIG. 1.
Schematic maps and the phenotypes summarized of the mutants analyzed in this study. Vectors are named as in Table I, as indicated with Footnote a. Footnote b shows the conserved region among DP1s that shows homology to the eukaryote small subunit of DNA polymerase is indicated in the box colored in yellow. The mini-intein insertion site and the catalytic center for polymerization in DP2 are shown with arrows and vertical lines. The region that shows homology to the catalytic subunit of yeast DNA polymerase in DP2 is indicated in the box colored in red. Two cysteine clusters are indicated with boxes filled with horizontal lines. The numbers in bold and blue in the parentheses are the numbers of peptide without the intein. Footnote c indicates the thermostability of the truncated peptide (ther. stab.), defined as the presence (+) or absence (-) of specific bands on SDS-GAGE analysis of the supernatant after heating at 85 °C for 30 min (Fig. 3). Subunit Inter., subunit interaction, the ability of complex formation between DP1Pho and DP2Pho after purification steps; Pol., polymerization; Exo., 3'-5' exonuclease activities; +, positive; -, negative; blank, not checked.