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Cobamides and Ribonucleotide Reduction

II. ESTIMATION OF THE ENZYMIC FORMATION OF PURINE AND PYRIMIDINE DEOXYRIBONUCLEOTIDES BY THE USE OF THE DIPHENYLAMINE REAGENT

From the ¹ From the Department of Biochemistry, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia

A colorimetric method is described for the estimation of purine and pyrimidine deoxyribonucleotides produced in the ribonucleotide reductase system. Conditions for the maximal production of color are described. The method enables the convenient determination of ribonucleotide reductase activity with a variety of substrates.

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