JBC DNA damage antibodies

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Comparative Study of Tadpole and Frog Glutamate Dehydrogenases

Barbara O. Wiggert 1 and Philip P. Cohen 1

From the 1 From the Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin 53706

1. There is approximately a 10-fold increase in the specific activity of liver glutamate dehydrogenase during the spontaneous metamorphosis of tadpoles (Rana catesbeiana).

2. Comparative studies of glutamate dehydrogenases from premetamorphic tadpole liver and adult frog liver revealed differences in kinetic constants for DPN and TPN, in the effect of temperature on initial rates with DPN as coenzyme, in the crude molecular weights estimated from sucrose density centrifugation, and in relative reactivities with l-amino acids other than glutamate.

3. An antibody produced in rabbits against crystalline frog liver glutamate dehydrogenase precipitates glutamate dehydrogenase preparations from frog, tadpole, beef, and chicken liver and affects their reaction kinetics in a similar manner.

Submitted on June 28, 1965


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P. P. Cohen
Biochemical Differentiation during Amphibian Metamorphosis
Science, May 1, 1970; 168(3931): 533 - 543.
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