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Biochemical Studies on Inositol

IX. d-INOSITOL 1-PHOSPHATE AS INTERMEDIATE IN THE BIOSYNTHESIS OF INOSITOL FROM GLUCOSE 6-PHOSPHATE, AND CHARACTERISTICS OF TWO REACTIONS IN THIS BIOSYNTHESIS

From the ¹ From the Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

At least two enzymatic steps have been shown to participate in the biosynthesis of inositol from glucose 6-phosphate. The first one, catalyzed by glucose-6-P cyclase, leads to the formation of d-inositol-1-P and has an absolute requirement for diphosphopyridine nucleotide. NH₄⁺ accelerates the rate of this reaction 5-fold. In the second step, catalyzed by a phosphatase, d-inositol-1-P is hydrolyzed to inositol and inorganic orthophosphate. Mg⁺⁺ ions are necessary for this reaction.

The inositol phosphate formed in the cyclase reaction was isolated by ion exchange chromatography and was identified as d-inositol-1-P by paper chromatography, its molar ratio of inositol to phosphorus, and its specific optical rotation.

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