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Electron Carriers of the Bovine Adrenal Cortical Respiratory Chain and Hydroxylating Pathways

From the ¹ From the Departments of Biochemistry and Medicine, University of Southern California School of Medicine, Los Angeles, California 90053

Difference spectra of mitochondrial and microsomal preparations from bovine adrenal cortex have been examined, and the activity of several enzyme systems in these particles has been studied. Evidence for nicotinamide adenine dinucleotide, flavoproteins, and cytochromes b, c₁, c, and a + a₃ in the adrenal respiratory chain in a molar ratio of 4.9:3.8:1.7:1.5:2.0:2.9:1.0, respectively, was found. The P-450 cytochrome was observed to be present in mitochondria in a molar ratio to cytochrome a₃ of 6.9:1.0. It paralleled malate oxidase and steroid 11ß-hydroxylase activities in subcellular distribution but was also present at a lower concentration in microsomal particles which contained most of the steroid 21-hydroxylase activity.

The relationship of the adrenal cortical respiratory chain to the mitochondrial steroid-hydroxylating pathway is discussed, and a role for an energy-controlled pyridine nucleotide transhydrogenase in electron transport to the hydroxylating system is suggested.

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