HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Fessenden, J. M. Articles by With the technical assistance of M. Anne Dannenberg, Search for Related Content PubMed PubMed Citation Articles by Fessenden, J. M. Articles by With the technical assistance of M. Anne Dannenberg, Social Bookmarking                      What's this?

Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation

XI. STIMULATION OF OXIDATIVE PHOSPHORYLATION BY COUPLING FACTORS AND OLIGOMYCIN; INHIBITION BY AN ANTIBODY AGAINST COUPLING FACTOR 1

From the ¹ From the Department of Biochemistry, The Public Health Research Institute of the City of New York, Inc., New York, New York 10009

1. The observation that low concentrations of oligomycin stimulated oxidative phosphorylation in A-particles has been confirmed and extended.

2. Oligomycin was found to stimulate each of the three sites of oxidative phosphorylation, but optimal phosphorylation rates were obtained only when coupling factors were added as well. The P:O ratio with succinate was raised from 0.06 with particles alone to over 1.5 when coupling factors 1, 2, and 4, oligomycin, and thioglycerol were added.

3. The relatively high rates of oxidative phosphorylation in the presence of oligomycin or of any pair of coupling factors suggested that residual amounts of coupling factors were present in A-particles.

4. This suggestion was borne out by studies with an antibody against coupling factor 1, which inhibited ATPase, ³²P_i-ATP exchange, and oxidative phosphorylation at all three sites in submitochondrial particles. The stimulation of oxidative phosphorylation by oligomycin in A-particles was abolished by the antibody against F₁.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?