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The Fixation of Hydrocyanic Acid by a Psychrophilic Basidiomycete

From the ¹ From the Department of Botany and Microbiology, Montana State University, Bozeman, Montana 59715

-Aminopropionitrile-1-¹⁴C was identified in cultures of a psychrophilic basidiomycete fed H¹⁴CN. The results of the study suggest that this compound is a precursor of alanine. This suggestion is supported by experiments with H¹⁴C¹⁵N as well as by a time course evaluation of ¹⁴C labeling in -aminopropionitrile and alanine in fungal mats. Evidence is presented for the presence of a nitrile synthetase in cell-free extracts of this fungus. The enzyme catalyzes the condensation reaction of acetaldehyde, ammonia, and hydrogen cyanide to form -aminopropionitrile.

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