The Occurrence of a Glucose-Enzyme Complex in the Reaction Catalyzed by Sucrose Phosphorylase
Judith Voet 1 and Robert H. Abeles 1
From the
1 From the Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154
Evidence has been obtained for the formation of a glucose-enzyme complex in reactions catalyzed by sucrose phosphorylase isolated from Pseudomonas saccharophila. When sucrose phosphorylase is denatured after exposure to uniformly labeled sucrose-14C, the denatured protein contains firmly bound d-glucose or a compound derived from the d-glucose moiety of sucrose. The d-fructose moiety of sucrose is not bound to the protein.
Submitted on March 22, 1966