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The Occurrence of a Glucose-Enzyme Complex in the Reaction Catalyzed by Sucrose Phosphorylase

Judith Voet 1 and Robert H. Abeles 1

From the 1 From the Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154

Evidence has been obtained for the formation of a glucose-enzyme complex in reactions catalyzed by sucrose phosphorylase isolated from Pseudomonas saccharophila. When sucrose phosphorylase is denatured after exposure to uniformly labeled sucrose-14C, the denatured protein contains firmly bound d-glucose or a compound derived from the d-glucose moiety of sucrose. The d-fructose moiety of sucrose is not bound to the protein.

Submitted on March 22, 1966


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Appl. Environ. Microbiol.Home page
M. I. Trindade, V. R. Abratt, and S. J. Reid
Induction of Sucrose Utilization Genes from Bifidobacterium lactis by Sucrose and Raffinose
Appl. Envir. Microbiol., January 1, 2003; 69(1): 24 - 32.
[Abstract] [Full Text] [PDF]




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