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Isolation and Characteristics of Flavodoxin from Nitrogen-fixing Clostridium pasteurianum

From the ¹ From the Central Research Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898

The purification, crystallization, and biological activity of flavodoxin is reported. It is a new electron transport protein and has been isolated from extracts of cells of Clostridium pasteurianum grown on nitrogen gas or ammonia and in an iron-deficient medium. Flavodoxin forms yellow crystals from ammonium sulfate solution, has a molecular weight of 14,600, absorption maxima of the oxidized form at 272, 372, 443 mµ with a shoulder at 472 mµ, and contains no iron, molybdenum, cobalt, or labile sulfur. In equimolar amounts, it will substitute for ferredoxin in the metabolism of hydrogen and pyruvate and in the fixation of nitrogen by extracts of C. pasteurianum with an effectiveness of 70, 40, and 30%, respectively. It is suggested that flavodoxin replaces ferredoxin as an electron carrier in cells of C. pasteurianum grown in an iron-deficient medium since these cells contain less than 5% of the ferredoxin found in cells grown in an iron-sufficient medium.

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