HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bruton, J. Articles by Horner, W. H. Search for Related Content PubMed PubMed Citation Articles by Bruton, J. Articles by Horner, W. H. Social Bookmarking                      What's this?

Biosynthesis of Streptomycin

III. ORIGIN OF THE CARBON ATOMS OF STREPTOSE

From the ¹ From the Department of Biochemistry, Schools of Medicine and Dentistry, Georgetown University, Washington D. C. 20007

The pathway of synthesis by Streptomyces griseus of the streptose moiety of streptomycin from D-glucose has been studied. L-Serine-3-¹⁴C, D-glucose-l-¹⁴C, D-glucose-2-¹⁴C, D-glucose-3,4-¹⁴C, and D-glucose-6-¹⁴C were given to streptomycin-producing cultures. A 40 to 46% incorporation of ¹⁴C into the streptose moiety of the isolated streptomycin was found. Procedures for the total degradation of streptose were developed and utilized to determine the ¹⁴C-label pattern in this moiety. Examination of the isotope distribution data for the 6 carbons of streptose after administration of the above labeled compounds to the organism shows that streptose arises from a carbon-carbon rearrangement at carbon atoms 3 and 4 of glucose to form the branched sugar, streptose.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?