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The Biosynthesis of Cell Wall Lipopolysaccharide in Escherichia coli

IV. PURIFICATION AND PROPERTIES OF CYTIDINE MONOPHOSPHATE 3-DEOXY-D-MANNO-OCTULOSONATE SYNTHETASE

Mohammad Ali Ghalambor 1 and Edward C. Heath 1

From the 1 From the Rackham Arthritis Research Unit and Department of Microbiology, The University of Michigan, Ann Arbor, Michigan, and the Department of Physiological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Cytidine monophosphate 3-deoxy-D-manno-octulosonate synthetase catalyzes the reversible formation of cytidine monophosphate 3-deoxy-D-manno-octulosonate from cytidine triphosphate and 3-deoxy-D-manno-octulosonate. The synthetase has been purified approximately 170-fold from crude extracts of Escherichia coli 0111-B4. The purified enzyme exhibits an absolute requirement for a divalent cation (Mg++) and is specific for cytidine triphosphate (Km = 2.2 x 10-4 M) and 3-deoxy-D-manno-octulosonate (Km = 8 x 10-4 M).

Submitted on January 21, 1966


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