HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mintel, R. Articles by Westley, J. Search for Related Content PubMed PubMed Citation Articles by Mintel, R. Articles by Westley, J. Social Bookmarking                      What's this?

The Rhodanese Reaction

MECHANISM OF SULFUR-SULFUR BOND CLEAVAGE

From the ¹ From the Department of Biochemistry, The University of Chicago, Chicago, Illinois 60637

Kinetic studies comparing methanethiosulfonate and thiosulfate as rhodanese substrates have shown that the cleavage of the thiosulfate sulfur-sulfur bond limits the over-all maximum velocity of the thiosulfate-cyanide sulfur transfer reaction. A kinetically significant enzyme-thiosulfate complex has thus been shown. The same studies have indicated that the Michaelis constant for thiosulfate is a true dissociation constant for this complex. Infrared spectral studies and experiments involving the reaction between thiosulfonates and cyanide in nonenzymic systems have shown that the reactivity of the planetary sulfur atom toward cyanide is enhanced by a shift of electrons away from the sulfur-sulfur bond. Studies of the enzyme-catalyzed reaction between the thiosulfonates and cyanide have suggested that one aspect of enzymic catalysis in the thiosulfate reaction is such a shift of electrons away from the sulfur-sulfur bond. A basis for the anomalous behavior of aromatic substrates has been discussed.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Guan, M. Sahin-Toth, T. Kalai, K. Hideg, and H. R. Kaback Probing the Mechanism of a Membrane Transport Protein with Affinity Inactivators J. Biol. Chem., March 14, 2003; 278(12): 10641 - 10648. [Abstract] [Full Text] [PDF]

				A. M. Westley and J. Westley Enzyme Inhibition in Open Systems J. Biol. Chem., March 8, 1996; 271(10): 5347 - 5352. [Abstract] [Full Text] [PDF]