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The Rhodanese Reaction

MECHANISM OF THIOSULFATE BINDING

From the ¹ From the Department of Biochemistry, The University of Chicago, Chicago, Illinois 60637

The interaction of rhodanese with thiosulfate to form a rhodanese-thiosulfate complex has been found to involve charge neutralization as evidenced by solvent and salt effects on the Michaelis constant in the absence of such effects on the maximum velocity. It is inferred from these findings that a cationic binding group for thiosulfate is present at the active site.

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