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Preparation of a Highly Purified Phenylalanine Transfer Ribonucleic Acid

From the ¹ From the Chemical Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831

A large sample of highly purified phenylalanine transfer ribonucleic acid from Escherichia coli B was prepared by reversed phase chromatography followed by gel filtration to remove ribonucleic acid contaminants of higher molecular weight. The phenylalanine acceptance and terminal adenosine content of this sample were identical, indicating that the sample was all active phenylalanine transfer ribonucleic acid. An independent measurement of purity obtained on a weight basis gave a minimum value of 83%. Preliminary data indicate that the phenylalanine transfer ribonucleic acid molecular weight is approximately 26,000.

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