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Subtilisin BPN'

IV. ISOLATION AND SEQUENCE OF TWO ARGININE-CONTAINING PEPTIDES

From the ¹ From the Department of Biological Chemistry, U.C.L.A School of Medicine, University of California, Los Angeles, California 90024

A tryptic digest of diisopropylphosphoryl-subtilisin BPN' was fractionated on a column of Dowex 1, and the two arginine-containing peptides were isolated. The sequences of a decapeptide and a hexadecapeptide are given. With these 26 residues added to 178 already reported, 204 of the 280 residues of the protein have been obtained in tryptic peptides.

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