HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Glazer, A. N. Search for Related Content PubMed PubMed Citation Articles by Glazer, A. N. Social Bookmarking                      What's this?

Transesterification Reactions Catalyzed by Papain

From the ¹ From the Department of Biological Chemistry, School of Medicine, University of California, Los Angeles, California 90024

The hydrolysis of a series of esters of benzoyl-L-arginine and glycylglycine by papain has been examined in mixtures of water with various alcohols in the pH range of 5 to 6. The presence of straight chain alcohols resulted in an apparent inhibition of acid release. It was shown that this inhibition could, in part, be accounted for by papain-catalyzed transesterification reactions between the various substrates and the alcohols studied. The products of transesterification, transamidation, and hydrolysis resulting on incubation of papain with esters of glycylglycine were separated and identified, and the kinetics of their formation were examined. Ficin was found to behave similarly to papain. The present study, in conjunction with earlier reports, suggests that the ability to catalyze alcoholytic reactions may be a property of many hydrolytic enzymes.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?