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Phospholipid-Metal Complexes

INTERACTION OF TRIPHOSPHOINOSITIDE- AND PHOSPHATIDYLSERINE-METAL COMPLEXES WITH ETHYLENEDIAMINE, POLYAMINOACIDS, AND PROTEIN

From the ¹ From the Western Regional Research Laboratory, Western Utilization Research and Development Division, Agricultural Research Service, United States Department of Agriculture, Albany, California 94710

When ovalbumin, poly-l-lysine, poly-l-aspartic acid, or ethylenediamine is added to a biphasic chloroform-methanol-water system containing the Mg(II), Ni(II), or Ca(II) complex of triphosphoinositide or phosphatidylserine, mixed complexes are formed as evidenced by the formation of an interfacial precipitate similar to that observed by Dawson containing phospholipid, metal, and protein or polyamino acid, or by the presence of ethylenediamine in the chloroform-rich phase where it is ordinarily insoluble. Stable mixed complex formation was shown by gel filtration studies. Analysis of complexes before and after gel filtration indicates stable binding of one ethylenediamine per phospholipidmetal unit and weak binding of an additional molecule of the amine. Polylysine is bound to the triphosphoinositide-Ni(II) complex in a 0.64:1 ratio on a weight basis.

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