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From the
1 From the Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110
Under appropriate conditions, the activity of phosphofructokinase of skeletal muscle from frog and mouse is extremely sensitive to small changes in pH in the physiological range, a low pH decreasing the affinity of the enzyme for fructose 6-phosphate. It is concluded that shifts in intracellular pH are important in the regulation of phosphofructokinase, but that this effect makes interpretation of data from intact muscle quite difficult.
Effect of pH on the Kinetics of Frog Muscle Phosphofructokinase
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