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The Conversion of Carbon Dioxide to Acetate

II. THE ROLE OF -KETOISOVALERATE IN THE SYNTHESIS OF ACETATE BY CLOSTRIDIUM THERMOACETICUM

From the ¹ From the Laboratory of Biochemistry, Section on Enzymes, National Heart Institute, National Institutes of Health, Bethesda, Maryland 20014

When cell-free extracts of Clostridium thermoaceticum are incubated with pyruvate and ¹⁴CO₂, radioactive carbon is incorporated into the carboxyl groups of pyruvate, acetate, formate, and an unidentified fatty acid. The unknown acid was isolated and identified as -ketoisovalerate.

A metabolic pathway was proposed to explain the formation of -ketoisovalerate and the ability of C. thermoaceticum to ferment glucose to nearly 3 eq of acetate. Data are presented showing that -ketoisovalerate is probably not an intermediate in the total synthesis of acetate from CO₂ which is catalyzed by C. thermoaceticum. However, the evidence suggests that the proposed pathway of metabolism might account in part for the ability of this organism to catalyze incorporation of CO₂ into the carboxyl group of acetate.

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