The Separation of New Forms of the Proteinpolysaccharides of Bovine Nasal Cartilage
Subhash Pal 1, Paul T. Doganges 1, and Maxwell Schubert 1
From the
1 From the Department of Medicine, the Study Group for Aging, and the Study Group for Rheumatic Diseases, New York University School of Medicine, New York, N. Y. 10016
The principal proteinpolysaccharide, called PP-L, of bovine nasal cartilage has been fractionated centrifugally to give four products. The method requires low concentration of PP-L, high concentration of salt, and makes use of a remarkable difference between the effects of salts with monovalent and divalent cations on the sedimentation behavior of these proteinpolysaccharides. A fifth product, PP-L2, has previously been isolated from cartilage residue. Of these five, three are almost identical analytically, but differ in the stage of the whole procedure at which their separation becomes possible. The protein contents of the five lie in the range 9 to 36%, but the amino acid profiles of all five are very similar. Of the five forms, the one called PP-L3 behaves in the analytical ultracentrifuge as a single component; the others do not. The two forms with high protein contents sediment most easily and have the highest limiting viscosity numbers. These five products account for 40% of the original dry cartilage weight and 85% of its uronic acid content.
Submitted on April 20, 1966
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