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Mixed Function Oxidation

VI. PURIFICATION OF A TIGHTLY COUPLED ELECTRON TRANSPORT COMPLEX IN CAMPHOR LACTONIZATION

From the ¹ From the Biochemistry Division, Chemistry Department, University of Illinois, Urbana, Illinois 61801

A multimeric ketolactonizing enzyme system has been purified 10-fold from (+)-camphor-grown Pseudomonas putida, and contains flavin mononucleotide and flavin adenine dinucleotide. Electron transport in the system is tightly controlled and unitary stoichiometry of reactants and products has been shown. A kinetic assay for the reaction has been developed, and the reversible reduction of flavin during catalysis was investigated.

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