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Substrate Inhibition with Glucose Oxidase

From the ¹ From the Department of Chemistry, Purdue University, Lafayette, Indiana 47907

The currently accepted mechanism for the action of glucose oxidase does not allow for substrate inhibition; on the other hand, a very similar flavin enzyme, l-amino acid oxidase, does show pronounced substrate inhibition. Experiments reported here done at very low oxygen levels show that glucose also inhibits the glucose oxidase reaction, and the probability is that, rather than each being unique, these two flavin enzymes operate by way of the same basic mechanism, the differences being in the relative values of the rate constants for the reactions associated with the mechanism.

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