JBC Ideal method for primary cell transfection

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Phosphatase-free Crystalline Micrococcal Nuclease

E. Sulkowski 1 and M. Laskowski Sr. 1

From the 1 From the Laboratory of Enzymology, Roswell Park Memorial Institute, Buffalo, New York 14203

This report relates to the preceding paper by Taniuchi and Anfinsen. Micrococcal nuclease from the strain "Foggi" has been purified from a commercially available source (Worthington). The enzyme was crystallized from an ammonium sulfate solution. The over-all purification was approximately 500-fold with an over-all yield of about 16%. The enzyme is virtually devoid of acid and alkaline phosphatases.

Submitted on May 2, 1966


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