Leucine Aminotransferase

I. COLORIMETRIC ASSAYS

From the ¹ From the Department of Biochemistry, University of California, Berkeley, California 94720

A simple colorimetric assay for measuring leucine--ketoglutarate transamination is described. It is reproducible, sensitive, and specific for -ketoisocaproate. -Ketoglutarate interferes only in that high concentrations decrease the color yield. This assay is suitable for detailed kinetic studies, even with crude tissue extracts, provided one corrects for this effect of -ketoglutarate. A separate method was also described whereby transamination between isoleucine (or valine) and -ketoglutarate can be measured, although this assay is not specific for the leucine transaminase in crude tissue extracts. The -ketoisocaproate assay was used to examine subcellular fractions of pig heart muscle. Most of the activity was found in the soluble fraction.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. R. Christopherson, G. E. Schmitz, and D. M. Downs YjgF Is Required for Isoleucine Biosynthesis when Salmonella enterica Is Grown on Pyruvate Medium J. Bacteriol., April 15, 2008; 190(8): 3057 - 3062. [Abstract] [Full Text] [PDF]

				M. G. Bixel, S. M. Hutson, and B. Hamprecht Cellular Distribution of Branched-chain Amino Acid Aminotransferase Isoenzymes Among Rat Brain Glial Cells in Culture J. Histochem. Cytochem., May 1, 1997; 45(5): 685 - 694. [Abstract] [Full Text] [PDF]