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An Effect of Puromycin on the Synthesis of Collagen by Embryonic Cartilage in Vitro

From the ¹ From the Departments of Medicine and Biochemistry, University of Pennsylvania, and the Philadelphia General Hospital, Philadelphia, Pennsylvania 19104

Large concentrations of puromycin inhibited the synthesis of peptide-bound hydroxyproline by embryonic cartilage to a greater extent than they inhibited the synthesis of polypeptides. Investigation of this effect provided further evidence for the suggestion that hydroxyproline is synthesized by the hydroxylation of proline after proline is incorporated into a proline-rich polypeptide precursor of collagen. Gel filtration of the soluble polypeptides which were obtained from samples inhibited with puromycin suggested that polypeptides with molecular weights greater than 10,000 must be synthesized before the hydroxylation can occur.

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				R. S. Bhatnagar, D. J. Prockop, and J. Rosenbloom Intracellular Pool of Unhydroxylated Polypeptide Precursors of Collagen Science, October 27, 1967; 158(3800): 492 - 494. [Abstract] [PDF]