The Effects of Histones and Other Polycations on Cellular Energetics
IV. FURTHER STUDIES ON THE STIMULATION OF MITOCHONDRIAL OXYGEN CONSUMPTION
Carl L. Johnson 1, Brian Safer 1, and Arnold Schwartz 1
From the
1 From the Department of Pharmacology, Baylor University College of Medicine, Houston, Texas 77025
The histone f2a was found to stimulate markedly oxygen consumption of isolated heart and liver mitochondria, in the absence of a phosphate acceptor system, but in the presence of inorganic phosphate. Octylguanidine, but not oligomycin or aurovertin, markedly inhibited the histone-induced increment in respiration. On the other hand the histone stimulated oligomycin- or aurovertin-inhibited respiration, but had no effect on respiration inhibited by octylguanidine. The stimulation of oxygen consumption by f2a was accompanied by an energy-dependent K+ efflux.
Submitted on February 17, 1966