HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Avigad, G. Articles by Pifko, S. Search for Related Content PubMed PubMed Citation Articles by Avigad, G. Articles by Pifko, S. Social Bookmarking                      What's this?

5-Keto-d-fructose

IV. A SPECIFIC REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE-LINKED REDUCTASE FROM GLUCONOBACTER CERINUS

From the ¹ From the Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel, and the Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, New York, New York 10461

A specific reduced nicotinamide adenine dinucleotide phosphate-linked 5-keto-d-fructose reductase has been isolated and purified approximately 1200-fold from extracts of Gluconobacter cerinus. The reaction catalyzed was found to be 5-Keto-d-fructose + NADPH + H⁺ d-fructose + NADP⁺ with an equilibrium far towards the right (K_eq = 2 x 10¹⁰). K_m values for NADPH, NADP⁺, 5-ketofructose, and fructose were 1.8 x 10^-6 m; 1.3 x 10^-3 m; 4.5 x 10^-3 m; and 7.0 x 10^-2 m, respectively, at pH 7.4.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?