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The Subunit Structure of Myosin

I. POLYDISPERSITY IN 5 m GUANIDINE

P. Dreizen 1, D. J. Hartshorne 1, and A. Stracher 1

From the 1 From the Departments of Medicine and Biochemistry, State University of New York Downstate Medical Center, Brooklyn 3, New York

Preparations of rabbit skeletal myosin that are reduced and carboxymethylated in 5 m guanidine-HCl-0.4 m KCl were found to be polydisperse, having a low molecular weight component of the order of 46,000 comprising approximately 17% of the protein, and a heavier fraction with a molecular weight greater than 200,000.

Submitted on May 28, 1965


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