HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moore, E. C. Articles by Hurlbert, R. B. Search for Related Content PubMed PubMed Citation Articles by Moore, E. C. Articles by Hurlbert, R. B. Social Bookmarking                      What's this?

Regulation of Mammalian Deoxyribonucleotide Biosynthesis by Nucleotides as Activators and Inhibitors

From the ¹ From the Department of Biochemistry, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

The effects of several nucleoside triphosphates as activators and inhibitors of the enzymic reduction of ribonucleoside diphosphates to deoxynucleotides was studied. The enzyme was a partially purified preparation of the ribonucleotide reductase system from the Novikoff ascites rat tumor, with dithioerythritol used as a model reducing agent. The reduction of cytidine diphosphate and uridine diphosphate to the corresponding deoxyribonucleotides required activation of the enzyme by adenosine triphosphate; reduction of guanosine diphosphate required activation by low concentrations of deoxythymidine triphosphate with ATP giving further stimulation; and reduction of ADP required activation by low concentrations of dGTP or dTTP. Reduction of CDP and UDP was inhibited by dTTP, dUTP, dGTP, and dATP; reduction of GDP was inhibited by dGTP and dATP; and reduction of ADP was inhibited by dATP. Only slight inhibition by dCTP was seen. The results are interpreted in terms of metabolic control of the biosynthesis of deoxynucleotides by these nucleoside triphosphates serving as regulatory effectors.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. D. Boucher, M. M. Im, S. O. Freytag, and D. S. Shewach A novel mechanism of synergistic cytotoxicity with 5-fluorocytosine and ganciclovir in double suicide gene therapy. Cancer Res., March 15, 2006; 66(6): 3230 - 3237. [Abstract] [Full Text] [PDF]

				R. W. Setzer, C. Lau, M. L. Mole, M. F. Copeland, J. M. Rogers, and R. J. Kavlock Toward a Biologically Based Dose-Response Model for Developmental Toxicity of 5-Fluorouracil in the Rat: A Mathematical Construct Toxicol. Sci., January 1, 2001; 59(1): 49 - 58. [Abstract] [Full Text] [PDF]

				C. Gran, A. Boyum, R. F. Johansen, D. Lovhaug, and E. C. Seeberg Growth Inhibition of Granulocyte-Macrophage Colony-Forming Cells by Human Cytidine Deaminase Requires the Catalytic Function of the Protein Blood, June 1, 1998; 91(11): 4127 - 4135. [Abstract] [Full Text] [PDF]

				M. Hershfield and N. Krodich S-adenosylhomocysteine hydrolase is an adenosine-binding protein: a target for adenosine toxicity Science, November 17, 1978; 202(4369): 757 - 760. [Abstract] [PDF]