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Measurement of Guanosine 3',5'-Monophosphate and Other Cyclic Nucleotides

VARIATIONS IN URINARY EXCRETION WITH HORMONAL STATE OF THE RAT

Joel G. Hardman 1, James W. Davis 1, and Earl W. Sutherland 1

From the 1 From the Department of Physiology, Vanderbilt University, Nashville, Tennessee 37203

A method has been developed for the determination of guanosine 3',5'-monophosphate, uridine 3',5'-monophosphate, and adenosine 3',5'-monophosphate by modifying one basic assay system. Applying this method to the measurement of urinary excretion of cyclic nucleotides in rats, guanosine 3',5'-monophosphate excretion was found to be decreased by hypophysectomy while adenosine 3',5'-monophosphate excretion was unchanged. Guanosine 3',5'-monophosphate excretion in hypophysectomized rats was elevated by combined treatment with hydrocortisone plus six pituitary hormones or with hydrocortisone plus thyroxine.

Submitted on August 1, 1966


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This article has been cited by other articles:


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E. W. Sutherland
Studies on the Mechanism of Hormone Action
Science, August 4, 1972; 177(4047): 401 - 408.
[PDF]

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J. Bourgoignie, S. Guggenheim, D. M. Kipnis, and S. Klahr
Cyclic Guanosine Monophosphate: Effects on Short-Circuit Current and Water Permeability
Science, September 26, 1969; 165(3900): 1362 - 1363.
[Abstract] [PDF]


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