HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thomas, P. J. Articles by Law, J. H. Search for Related Content PubMed Articles by Thomas, P. J. Articles by Law, J. H. Social Bookmarking                      What's this?

Biosynthesis of Cyclopropane Compounds

IX. STRUCTURAL AND STEREOCHEMICAL REQUIREMENTS FOR THE CYCLOPROPANE SYNTHETASE SUBSTRATE

From the ¹ From the James Bryant Conant Laboratory, Harvard University, Cambridge, Massachusetts 02138

Several natural and synthetic phospholipids can serve as substrates for the cyclopropane synthetase enzyme of Clostridium butyricum. The conditions for optimum substrate effectiveness vary with the substrate; e.g. an anionic surface-active agent is necessary with phosphatidylethanolamine, while divalent cations stimulate the rate of reaction with anionic phospholipids. These variable conditions probably provide the optimal surface charge on the phospholipid aggregates for most efficient enzyme-substrate interaction.

A diether analogue of phosphatidylethanolamine, 1,2-di(9-octadecenyloxy)-3-(2-aminoethyl)phosphorylpropane, is an effective substrate for cyclopropane synthetase, indicating that the ester carbonyl groups are not essential for activity.

When presented with a racemic mixture of dioleyl phosphatidylethanolamines, the enzyme acts nearly exclusively upon the 3-phosphoglycerol isomer.

Cyclopropane synthetase acts upon phospholipids deposited upon filter paper discs. The pattern of activity with various phospholipids is different from that with the compounds dispersed in buffer, and no accessory substances such as surface-active agents or divalent cations stimulate the reaction on filter paper.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				X. Bao, J. J. Thelen, G. Bonaventure, and J. B. Ohlrogge Characterization of Cyclopropane Fatty-acid Synthase from Sterculia foetida J. Biol. Chem., April 4, 2003; 278(15): 12846 - 12853. [Abstract] [Full Text] [PDF]