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Separation and Properties of Multiple Components of Bovine Growth Hormone

Charles A. Free 1 and Martin Sonenberg 1

From the 1 From the Division of Clinical Investigation, Sloan-Kettering Institute for Cancer Research, New York, New York 10021

Starch gel electrophoresis at alkaline pH of bovine growth hormone preparations from a number of sources has revealed a common pattern characterized by a major anionic component and two minor faster moving components. Gel filtration studies of the hormone indicate that all three components are of similar molecular weight. A method has been developed for separation of the components by chromatography on diethylaminoethyl Sephadex. All three components were biologically active and displayed similar amino acid compositions.

Submitted on June 6, 1966


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