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The Role of Transfer Ribonucleic Acid in the Pyrophosphate Exchange Reaction of Arginine-Transfer Ribonucleic Acid Synthetase

From the ¹ From the Department of Biochemistry, Marquette University School of Medicine, Milwaukee, Wisconsin 53233

An arginine-activating enzyme purified from Escherichia coli does not catalyze the exchange of pyrophosphate and adenosine triphosphate except in the presence of the transfer ribonucleic acid capable of accepting arginine. The back reaction, in which arginyl-tRNA reacts with adenylic acid and pyrophosphate, proceeds at about the same rate as the forward reaction. The back reaction is inhibited only slightly by components of the forward reaction, but the forward reaction is strongly inhibited by pyrophosphate. An isotope-trapping experiment showed that free arginyl-tRNA was not formed in the presence of pyrophosphate. It is suggested that tRNA serves as an activator of the arginine-activating enzyme.

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