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Pathway of Alginic Acid Synthesis in the Marine Brown Alga, Fucus gardneri Silva

From the ¹ From the Department of Biochemistry, University of California, Berkeley, California 94720

When discs of the marine brown alga, Fucus gardneri, were infiltrated with uniformly labeled d-mannose-¹⁴C or uniformly labeled d-glucose-¹⁴C, radioactivity was detected in the respiratory CO₂, "fucoidin," "alginic acid," and in the residual fractions. Radioactivity was also found in the ethanol and in the acid extracts of the leaves. The extracts contained sugar phosphates, sugar nucleotides, and glyconic acids.

Enzyme preparations were obtained from the alga which contained the following enzymic activities: hexokinase, phosphomannomutase, d-mannose 1-phosphate guanylyl-transferase, guanosine diphosphate-d-mannose dehydrogenase, and mannuronic acid transferase. It was shown that, starting with d-mannose, these enzyme systems are involved in the pathway leading to the formation of guanosine diphosphate-d-mannuronic acid and subsequent incorporation of the d-mannuronic acid into a polymer of the uronic acid.

The enzyme activities of the F. gardneri could be observed only when the cell-free preparations were made in the presence of polyvinylpyrrolidone.

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