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The Sialic Acids

IX. ISOLATION OF CYTIDINE 5'-MONOPHOSPHO-N-ACETYLNEURAMINIC ACID FROM ESCHERICHIA COLI K-235

From the ¹ From the Rackham Arthritis Research Unit and the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan

A novel type of sugar nucleotide, containing 1 rather than 2 phosphate residues, was isolated from Escherichia coli K-235. The nucleotide was shown to be cytidine 5'-monophospho-N-acetylneuraminic acid in which the sialic acid was glycosidically linked to the phosphate residue. The glycoside was highly sensitive to acid, and optical rotation studies showed that it was the ß anomer, in contrast to other known, natural, sialidase-sensitive sialic acid glycosides.

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