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Formation of -Ketoglutaric Semialdehyde from l-2-Keto-3-deoxyarabonic Acid and Isolation of l-2-Keto-3-deoxyarabonate Dehydratase from Pseudomonas saccharophila

From the ¹ From the Graduate Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48104, and the Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154

-Ketoglutaric semialdehyde has been identified as an intermediate in the conversion of l-2-keto-3-deoxyarabonate to -ketoglutarate by cell-free extracts of Pseudomonas saccharophila. -Ketoglutarate semialdehyde arises by the intramolecular dismutation of l-2-keto-3-deoxyarabonate. The enzyme, l-2-keto-3-deoxyarabonate dehydratase, which catalyzes the conversion of l-2-keto-3-deoxyarabonate to -ketoglutarate semialdehyde, has been purified and crystallized. The enzyme is homogeneous on starch gel electrophoresis and shows a single asymmetrical peak in the ultracentrifuge. No cofactor has been observed.

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