HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Turner, M. K. Articles by Lieberman, I. Search for Related Content PubMed PubMed Citation Articles by Turner, M. K. Articles by Lieberman, I. Social Bookmarking                      What's this?

Meso-,ß-diphenylsuccinate and Hydroxyurea as Inhibitors of Deoxycytidylate Synthesis in Extracts of Ehrlich Ascites and L Cells

From the ¹ From the Department of Biochemistry and Nutrition, Graduate School of Public Health, and the Department of Anatomy and Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15123

The formation of deoxycytidylate in soluble extracts of Ehrlich ascites cells grown in white Swiss mice and of L cells grown in monolayer cultures was shown to occur by ribonucleotide reduction without cleavage of the glycosidic bond. Meso-,ß-diphenylsuccinate, which had previously been described as a specific inhibitor for the activation of deoxyribose 5-phosphate aldolase, was found to block the nucleotide reductase reaction in these extracts. The inhibitory effectiveness was indistinguishable from that observed with hydroxyurea, and both agents affected the reduction reaction but not the kinases responsible for converting cytidine monophosphate to cytidine diphosphate and triphosphate.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?