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The Pathway of myo-Inositol Degradation in Aerobacter aerogenes

RING SCISSION

Thomas Berman 1 and Boris Magasanik 1

From the 1 From the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

The product of the first two reactions in the enzymatic degradation of myo-inositol by extracts of Aerobacter aerogenes, d-2,3-diketo-4-deoxy-epi-inositol, serves as the substrate for another enzyme also present in these extracts. The enzyme was partly purified and could be shown to catalyze, presumably after a preliminary isomerization, the hydrolytic cleavage of the inositol ring. The product of the reaction was isolated and identified by the study of its chemical and physical properties as a 4-deoxy-5-ketohexonic acid.

Submitted on July 15, 1965


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