HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sprecher, M. Articles by Sprinson, D. B. Search for Related Content PubMed PubMed Citation Articles by Sprecher, M. Articles by Sprinson, D. B. Social Bookmarking                      What's this?

Stereochemistry of the Glutamate Mutase Reaction

From the ¹ From the Departments of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, New York 10032, and University of California, Berkeley, California

4-Deuterioglutamic acid was prepared by incubation in D₂O of ammonium mesaconate with an extract of Clostridium tetanomorphum. In these extracts, methylaspartase catalyzed the formation of threo-3-methyl-l-aspartate-3-D, which was then rearranged to glutamate by the cobamide coenzyme-dependent glutamate mutase. The monodeuteriosuccinate obtained by chloramine-T oxidation of the glutamate showed a plain negative optical rotatory dispersion curve, and was therefore (R)-succinate-2-D. It was concluded that the intramolecular rearrangement of thero-3-methyl-l-aspartate to l-glutamate proceeded by net inversion of configuration of carbon atom 3. (R)-Succinate-2-D was also prepared from (2S,3R)-aspartic acid-3-D.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?