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Analyses of Large Oligonucleotide Fragments Obtained from a Yeast Alanine Transfer Ribonucleic Acid by Partial Digestion with Ribonuclease T1

From the ¹ From the United States Plant, Soil and Nutrition Laboratory, Soil and Water Conservation Research Division, Agricultural Research Service, United States Department of Agriculture, and Department of Biochemistry, Cornell University, Ithaca, New York

Procedures are described for the isolation of large oligonucleotide fragments after partial digestion of a yeast alanine transfer ribonucleic acid with Taka-Diastase ribonuclease T1. Quantitative analyses of the large fragments are presented. This paper completes the description of experimental methods used in the determination of the nucleotide sequence of this alanine transfer ribonucleic acid.

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