JBC INTERFERin siRNA transfection reagent

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Parathyroid Hormone and Bone Mobilization in Vitro

Alan Tenenhouse 1, Richard Meier 1, and Howard Rasmussen 1

From the 1 From the Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706

A two-phase system in vitro has been developed for studying the bone-mobilizing effects of parathyroid hormone. Phase I consists of the incubation of hormone with ascites cells, and Phase II is the incubation of Phase I supernatant with dead bone powder. The mobilization of labeled calcium from this powder is the index of hormonal activity. The response to hormone is apparent at 10-8 m hormone, appears specific for parathyroid hormone, and has striking analogies to hormone action upon living bone in vivo and in vitro.

Submitted on August 6, 1965


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