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Brain Mitochondria

V. INCORPORATION OF FATTY ACIDS INTO PHOSPHOLIPIDS IN BOVINE BRAIN MITOCHONDRIA

From the ¹ From the Biochemistry Department, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

The incorporation of labeled palmitate into phospholipids in bovine brain mitochondria was compared with fatty acid oxidation under various experimental conditions.

The rate of incorporation of palmitate into phospholipids proceeded at 20 to 25% of the rate of palmitate oxidation under optimal conditions and required exogenous adenosine triphosphate and coenzyme A. The addition of coenzyme A was not necessary for fatty acid oxidation.

In the presence of ATP, the incorporation into phospholipids was not affected by cyanide, dinitrophenol, or oligomycin, although these agents inhibited fatty acid oxidation. In the absence of exogenous nucleotides, oligomycin inhibited the incorporation of palmitate into phospholipids and stimulated palmitate oxidation.

A preincubation with glucose and hexokinase decreased the incorporation of palmitate into phospholipids to a greater extent than palmitate oxidation. The addition of oligomycin after the preincubation further inhibited the incorporation into phospholipids but reversed the inhibition of fatty acid oxidation.

These results indicate that in brain mitochondria ATP is necessary for the activation of fatty acids before incorporation into phospholipids and that high energy intermediates are not involved. It is suggested that the activation of fatty acids before oxidation or esterification may involve different energy sources and that these two metabolic processes may occur in separate compartments of the mitochondrion.

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