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Biochemical Studies of Bacterial Sporulation

II. DEOXYRIBONUCLEIC ACID POLYMERASE IN SPORES OF BACILLUS SUBTILIS

From the ¹ From the Department of Biochemistry, Stanford University School of Medicine, Palo Alto, California 94304

Extracts of Bacillus subtilis spores were shown to contain deoxyribonucleic acid polymerase with a specific activity comparable to that in extracts of vegetative cells. The enzyme was partially purified and proved to be indistinguishable by several physical and functional criteria from the isolated vegetative cell polymerase.

The isolated spore and vegetative cell enzymes are equally heat-labile, whereas inside the spore the polymerase is far more heat-stable than within the vegetative cell.

The bulk of polymerase activity disappears during sporulation, and only about 30% is recovered in the spores. It is inferred that there is loss or destruction of the residual polymerase. Whether polymerase molecules are newly synthesized for the spore or simply a legacy from the vegetative cell population remains to be determined.

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