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Augmentation of Aldolase Activity by High Concentration of Fructose 1,6-Diphosphate

From the ¹ From the Institute of Marine Resources, Department of Nutritional Sciences, University of California, Berkeley, California 94720

At high substrate (fructose 1,6-diphosphate) concentrations a 6-fold augmentation of aldolase activity was observed.

With freshly prepared aldolase the Michaelis constant was 3.5 x 10^-3 m and the molecular activity was 9.7 x 10² at lower substrate concentrations, whereas at higher substrate concentrations, those values were 6.3 x 10^-2 m and 5.8 x 10³, respectively.

The following equation was derived.

[see PDF for equation]

where n is the number of substrate molecules bound; a plot of log (1/v - 1/V') against log (S) indicated that there was a one to one substrate-enzyme interaction at low substrate concentrations but a two to one substrate-enzyme interaction at high substrate levels.

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