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Alterations in Protein and Nucleic Acid Metabolism of Thymocytes Produced by Adrenal Steroids in Vitro

From the ¹ From the Departments of Biochemistry and Pharmacology, Albert Einstein College of Medicine, Yeshiva University, New York, New York 10461

Thymocytes from cortisol-treated rats, incubated in vitro, showed a lower incorporation of radioactive precursors into ribonucleic acid, deoxyribonucleic acid, and protein than did cells from control animals. Thymolytic steroids present in vitro at concentrations of 10^-10 m to 10^-5 m in the thymocyte suspensions caused similar inhibitions. Good correlation was found between relative potency of the steroids tested in vitro and their reported potency assessed in vivo.

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